TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: PPFWE    
Protoporphyrins, Fractionation, Washed Erythrocytes

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test should not be ordered in conjunction with PEWE / Porphyrins Evaluation, Washed Erythrocytes.

 

The following algorithms are available in Special Instructions:

-Porphyria (Acute) Testing Algorithm

-Porphyria (Cutaneous) Testing Algorithm

Specimen Type Describes the specimen type validated for testing

Washed RBC

Advisory Information

This test is for assessment for protoporphyria, an erythropoietic porphyria. The preferred test for lead toxicity in children is blood lead. For more information see PBDV / Lead, Venous with Demographics, Blood or PBDC / Lead, Capillary, with Demographics, Blood. The preferred screening test for suspicion of a hepatic porphyria is urine porphyrins. For more information see PQNRU / Porphyrins, Quantitative, Random, Urine.

Necessary Information

1. Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen.

2. Include a list of medications the patient is currently taking.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

All porphyrin tests on erythrocytes can be performed on 1 tube.

 

Patient Preparation: Patient should abstain from alcohol for 24 hours.

Collection Container/Tube:

Preferred: Green top (sodium heparin)

Acceptable: Dark blue top (metal free heparin) or green top (lithium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: Washed erythrocyte suspension

Collection Instructions: Collect and process whole blood specimen as follows:

1. Transfer entire specimen to a 12-mL graduated centrifuge tube.

2. Centrifuge specimen for 10 minutes at 2000 rpm.

3. Record volume of packed cells and the total volume of the specimen.

4. Discard supernatant plasma.

5. Wash packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2000 rpm, discarding supernatant after each washing.

6. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.

7. Transfer washed erythrocytes into a plastic vial and freeze.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Forms

If not ordering electronically, complete, print, and send an Inborn Errors of Metabolism Test Request (T798) with the specimen. 

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Other Cell suspension not available

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Washed RBCFrozen (preferred)14 days
 Refrigerated 14 days