A short description of the method used to perform the test
Serum Neutralization Fluorescent Antibody
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Lists a shorter or abbreviated version of the Published Name for a test
Rabies RFFIT Endpoint, S
Lists additional common names for a test, as an aid in searching
Rapid Fluorescent Focus Inhib Test FORWARD
Describes the specimen type validated for testing
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Serum
Specimen Volume: 2 mL
Collection Instructions: Draw blood in a plain red-top tube(s), Spin down and send 2 mL of serum refrigerated in a plastic vial.
Note: 1. Serum gel tube is acceptable, but must be poured off into plastic vial.
2. Collection date is required.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis || Mild OK; Gross reject |
| Lipemia || Mild OK; Gross reject |
| Icterus || NA |
| Other || NA |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Reportable range is 0.1 to 15.0 IU/mL
Less than 0.1 IU/mL: Below detection limit
In humans a results of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance.
Provides information to assist in interpretation of the test results
Quantitative results. For those who want to know their exact titer between the reportable range.
RFFIT stands for Rapid Fluorescent Foci Inhibition Test. It is a serum neutralization (inhibition) test, which means it measures the ability of rabies specific antibodies to neutralize rabies virus and prevent the virus from infecting cells. The antibodies are called rabies virus neutralizing antibodies (RVNA).
Describes how the test is performed and provides a method-specific reference
In RFFIT, your serum (the non-cellular portion of your blood) is first diluted fivefold (1part serum in 4 parts diluent). Further (serial fivefold) dilutions are performed, each of which contain less and less of your serum. These serum dilutions are mixed with a standard amount of live rabies virus and incubated. Whatever RVNA is present will neutralize the virus. Next, some tissue culture cells are added and the serum/virus/cells are incubated together. Whatever rabies virus is left (i.e., that which has not been neutralized by the antibody in your serum), will infect the cells and this can be seen under the microscope through the use of specific staining. Calculation of the endpoint titer is made from the percent of virus infected cells observed on the slide.
Indicates whether the report includes an additional document with charts, images or other enriched information
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday, Wednesday, Thursday
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
3 to 5 weeks
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
RFFIT/K-State Rabies Laboratory Manhattan/K-State Innovation Center
Test Setup Resources
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.
Excel | Pdf
SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.
SI Normal Reports |
SI Abnormal Reports