Test Catalog

Test Id : VIBC

Vibrio Culture, Feces

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining whether Vibrio species may be the cause of diarrhea

 

This test is generally not useful for patients that have been hospitalized for more than 3 days because the yield from these patients' specimens is very low, as is the likelihood of identifying a pathogen that has not been detected previously

Highlights

This test provides evidence of the presence of the bacterium, Vibrio species, in feces, in a viable state, and provides an isolate for antibacterial susceptibility testing and, if needed, submission to a health department. Minnesota health care providers are required to report all confirmed or suspected cases of Vibrio bacteriosis to the Minnesota Department of Health. Clients should refer to their local health departments regarding public health submission of Vibrio isolates.

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
RMALD Ident by MALDI-TOF mass spec No, Bill Only No
GID Bacteria Identification No, Bill Only No
ISAE Aerobe Ident by Sequencing No, Bill Only No
REFID Additional Identification Procedure No, Bill Only No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged separately.

Method Name
A short description of the method used to perform the test

Conventional Culture

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Vibrio Culture, Stool

Aliases
Lists additional common names for a test, as an aid in searching

Enteric pathogens

Vibrio cholerae

Cholera

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged separately.

Specimen Type
Describes the specimen type validated for testing

Fecal

Additional Testing Requirements

1. If susceptibility testing is desired, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Vibrio species from feces), ZMMLS will not be performed and the order will be canceled.

2. In some cases, local public health requirements may impact Mayo Clinic Laboratories (MCL) clients, requiring submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Vibrio species recovered from specimens submitted to MCL by calling 800-533-1710 as soon as possible after reporting to ensure viability of the bacterium.

Shipping Instructions

Specimen must arrive within 96 hours of collection.

Necessary Information

Specimen source is required.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Patient Preparation: Medications: Do not use barium or bismuth before collection of specimen.

Supplies: C and S Vial (T058)

Specimen Type: Preserved feces

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Fecal swab Feces in Ecofix (green cap) Specimen in buffered glycerol saline transport media Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 4 days
Refrigerated 4 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining whether Vibrio species may be the cause of diarrhea

 

This test is generally not useful for patients that have been hospitalized for more than 3 days because the yield from these patients' specimens is very low, as is the likelihood of identifying a pathogen that has not been detected previously

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged separately.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Diarrhea may be caused by a number of agents (eg, bacteria, viruses, parasites, and chemicals), and infection with or exposure to one of these agents may result in similar symptoms. A thorough patient history covering symptoms, severity and duration of illness, age, travel history, food consumption, history of recent antibiotic use, and illnesses in the family or other contacts will help the physician determine the appropriate testing to be performed.

 

Vibrio cholerae, the causative agent of endemic, epidemic, and pandemic cholera, results in large volumes of rice-water stools due to the production of an enterotoxin. Severe dehydration is of concern in patients without access to adequate medical care. In the United States, Vibrio parahemolyticus is the most common cause of Vibrio disease. V parahemolyticus is associated with the consumption of raw shellfish or fish and results in gastroenteritis with nausea, vomiting, abdominal cramps, low-grade fever, and chills. Usually rehydration is the only treatment required, although in some cases, antimicrobial therapy is needed.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

No growth of pathogen

Interpretation
Provides information to assist in interpretation of the test results

The growth of Vibrio species identifies a potential cause of diarrhea.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

The yield of Vibrio species is reduced when specimens are delayed in transit to the laboratory (>2 hours from collection for unpreserved specimen).

 

Buffered glycerol saline is an unacceptable transport media for Vibrio culture.

 

Check your local public health requirements, which may require submission of isolates to a public health laboratory.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Pillai DR: Fecal culture for aerobic pathogens of gastroenteritis. In Clinical Microbiology Procedures Handbook, Fourth edition. Washington, DC, ASM Press, 2016, Section 3.8.1

2. Mandell GL, Bennett JE, Dolin R: Principle's and Practice of Infectious Disease. Sixth edition. Philadelphia, Elsevier Inc. 2005, pp 2536-2548

Method Description
Describes how the test is performed and provides a method-specific reference

The fecal specimen is inoculated onto selective media, thiosulfate-citrate-bile salts-sucrose agar (TCBS), designed to inhibit growth of normal bowel flora while allowing growth of Vibrio species. After incubation, suspect colonies are identified using 1 or a combination of the following techniques: matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, conventional biochemical tests, carbon source utilization, and nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene.(Tarr CL, Glover WAII, Boucher Y, et al: Chapter 43: Vibrio and Related Organisms. In Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller. Washington DC,ASM Press, 2019, pp 775-786)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

3 to 5 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87046-Vibrio culture, stool

87077-Identification by MALDI-TOF mass spec (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports