Test Catalog

Test Id : CCBF

Cell Count and Differential, Body Fluid

Useful For
Suggests clinical disorders or settings where the test may be helpful

Aids in the diagnosis of joint disease, systemic disease, inflammation, malignancy, infection, and trauma, using body fluid specimens

Profile Information
A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test Id Reporting Name Available Separately Always Performed
CCBF_ Cell Count and Differential, BF No Yes
CRSF Morphologic Review, BF No Yes

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
LCMS Leukemia/Lymphoma, Phenotype Yes No
CYTNG Cytology Non-GYN Yes No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When abnormal cytologic features are present, the laboratory may reflex to a miscellaneous cytology test. Fee codes for that test vary depending on review process.

Method Name
A short description of the method used to perform the test

Automated or Manual Cell Count/Cytocentrifugation followed by Manual Differential and Morphology Review

 

Stain slide with Wright-Giemsa stain.

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

No

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Cell Count and Differential, BF

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When abnormal cytologic features are present, the laboratory may reflex to a miscellaneous cytology test. Fee codes for that test vary depending on review process.

Specimen Type
Describes the specimen type validated for testing

Body Fluid

Ordering Guidance

For spinal fluid specimens, order CCCF / Cell Count and Differential, Spinal Fluid.

 

For bronchoalveolar lavage specimens, order LAV / Cell Count and Differential, Bronchoalveolar Lavage.

Shipping Instructions

Specimen must arrive within 24 hours of collection.

Necessary Information

Indicate specimen source.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
FLD2 Fluid Type

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

For Local Accounts Only

 

Sources: Synovial, pleural, peritoneal, pericardial

Container/Tube:

Preferred: Body fluid container

Acceptable: EDTA or heparin

Specimen Volume: 1 mL

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.7 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis OK
Clotted Nasal fluid, sputum, amniotic fluid Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Body Fluid Ambient (preferred) 24 hours
Refrigerated 24 hours

Useful For
Suggests clinical disorders or settings where the test may be helpful

Aids in the diagnosis of joint disease, systemic disease, inflammation, malignancy, infection, and trauma, using body fluid specimens

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When abnormal cytologic features are present, the laboratory may reflex to a miscellaneous cytology test. Fee codes for that test vary depending on review process.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Body fluids, other than the commonly analyzed urine and blood, include synovial, pleural, peritoneal, and pericardial fluids. These fluids may be present in increased volumes and may contain increased numbers of normal and abnormal cells in a variety of disease states.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

TOTAL NUCLEATED CELLS

Synovial fluid: <150/mcL

Peritoneal/pleural/pericardial fluid: <500/mcL

 

NEUTROPHILS

Synovial Fluid: <25%

Peritoneal/pleural/pericardial fluid: <25%

 

LYMPHOCYTES

Synovial fluid: <75%

 

MONOCYTES/MACROPHAGES

Synovial fluid: < or =70%

Interpretation
Provides information to assist in interpretation of the test results

Trauma and hemorrhage may result in increased red and white blood cells; RBCs predominate. WBCs are increased in inflammatory and infectious processes:

-Neutrophils predominate in bacterial infections

-Lymphocytes predominate in viral infections

-Macrophages may be increased in inflammatory and infectious processes

-Eosinophils may be increased in parasitic or fungal infections

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Kjeldsberg C, Knight J: Body Fluids: Laboratory examination of cerebrospinal, seminal, serous and synovial fluids. American Society of Clinical Pathologists, 1993

2. Dyken PR, Shirley S, Trefz J, El Gammel T: Comparison of cyto-centrifugation and sedimentation techniques for CSF cyto-morphology. Acta Cytologica 1980;20:137-170

3. Sheth KV: Cerebrospinal and body fluid cell morphology through a hematologist's microscope, workshop presented at the ASCP-CAP Joint Spring Meeting, San Diego, March 1981

4. Schumacher AH, Reginato A: Atlas of Synovial Fluid Analysis and Crystal Identification. Lea and Febiger, 1991

5. Hussong JW, Kjeldsberg CR: Kjeldsberg's Body Fluid Analysis. First edition. ASCP Press 2015

Method Description
Describes how the test is performed and provides a method-specific reference

The cells are applied to a glass slide by cytocentrifugation. Wright-Giemsa stained slides are examined by light microscopy and a differential is performed. Total nucleated cell count is determined using an automated hematology analyzer or a microscopic counting chamber. (Instruction manual: Automated Hematology Analyzer XN series [XN-1000]. Code No. CJ410539. North American Edition. November 2015)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 2 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

89051-Cell count with differential count

88184 if applicable

88185 if applicable

88187 if applicable

88188 if applicable

88189 if applicable

88104 if applicable

88108 if applicable

88112 if applicable

88161 if applicable

88162 if applicable

88305 if applicable

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
CCBF Cell Count and Differential, BF 34557-9
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
FLD2 Fluid Type 14725-6
APP2 Gross Appearance 9335-1
TOT12 Total Nucleated Cells 74689-1
RBC1 Erythrocytes 26455-6
CMT37 Comment 48767-8
NE_BF Neutrophils 26513-2
LY_BF Lymphocytes 11031-2
MM_BF Monocytes/Macrophages 30437-8
EO_BF Eosinophils 26452-3
BA_BF Basophils 28543-7
OTH1 Other Cells 75353-3
CMT3 Diff Comments 59466-3
DCCBF Download CCBF No LOINC Needed
OTH11 Other Cells Are: 75353-3
CMT81 Comment 48767-8
REV81 Reviewed by: 18771-6

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports