Aiding in the diagnosis of Eastern equine encephalitis
See Mosquito-borne Disease Laboratory Testing in Special Instructions.
Immunofluorescence Assay (IFA)
Arbovirus Serology
Eastern Equine Encephalitis (EEE)
EEE (Eastern Equine Encephalitis)
Encephalitis Antibodies
See Mosquito-borne Disease Laboratory Testing in Special Instructions.
Serum
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
0.15 mL
Gross hemolysis | Reject |
Gross lipemia | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 14 days |
Aiding in the diagnosis of Eastern equine encephalitis
See Mosquito-borne Disease Laboratory Testing in Special Instructions.
Eastern equine encephalitis (EEE) is within the alphavirus group.
Central nervous system involvement is demonstrated in only a minority of infected individuals; it is more abrupt and more severe with EEE than other arboviruses, with children being more susceptible to severe disease. Fatality rates are approximately 70% for EEE.
IgG: <1:10
IgM: <1:10
Reference values apply to all ages.
In patients infected with this virus, IgG antibody is generally detectable within 1 to 3 weeks of onset, peaking within 1 to 2 months, and declining
IgM class antibody is also reliably detected within 1 to 3 weeks of onset, peaking and rapidly declining within 3 months.
Single serum specimen IgG greater than or equal to 1:10 indicates exposure to the virus.
Results from a single serum specimen can differentiate early (acute)
A 4-fold or greater rise in IgG antibody titer in acute and
In the
Infections can occur at any age. The age distribution depends on the
All results must be correlated with clinical history and other data
Specimens collected within the first 2 weeks after onset are variably negative for IgG antibody and should not be used to exclude the diagnosis of arboviral disease. If arboviral infection is suspected, a second specimen should be collected and tested 10 to 21 days later.
Since cross-reactivity with dengue fever virus does occur with St.
Eastern equine encephalitis and Western equine encephalitis viruses show some cross-reactivity; however,
Usually, when an infection with an arbovirus is suspected, it is too late to
1. Gonzalez-Scarano F, Nathanson N: Bunyaviruses. In: Fields BN, Knipe DM, eds. Fields Virology. Vol 1. 2nd ed. Raven Press, 1990; 1195-1228
2. Donat JF, Hable-Rhodes KH, Groover RV, Smith TF: Etiology and outcome in 42 children with acute nonbacterial meningoencephalitis. Mayo Clin Proc. 1980;55:156-160
3. Tsai TF: Arboviruses. In: Murray PR, Baron EJ, Pfaller MA, et al, eds. Manual of Clinical Microbiology. 7th ed. American Society for Microbiology, 1999; 1107-1124
4. Calisher CH: Medically important arboviruses of the United States and Canada. Clin Microbiol Rev. 1994;7:89-116
5. Markoff L: Alphaviruses (Chikungunya, Eastern equine encephalitis). In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:1997-2006
Dilutions of test sera are prepared and allowed to react with substrate cells infected with Eastern equine encephalitis virus. If IgG antibodies to this virus are present in the serum of the patient, an antigen-antibody complex will develop that can be detected by a fluorescein-labeled antibody directed to human globulin.(Tsai TF: Arboviruses. In: Murray PR, Baron EJ, Pfaller MA, et al, eds. Manual of Clinical Microbiology. 7th ed. ASM Press, 1999; 1107-1124; Beaty BJ, Casals J, Brown KL, et al: Indirect fluorescent-antibody technique for serological diagnosis of LaCrosse [California] virus infections. J Clin Microbiol. 1982;15:429-434; Beckham JD, Tyler KL: Arbovirus Infections. Continuum (Minneap Minn). 2015 Dec;21(6 Neuroinfectious Disease):1599-1611. doi: 10.1212/CON.0000000000000240)
May through October: Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
86652 x 2
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
EEEP | East Equine Enceph Ab, IgG and IgM, S | 69034-7 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
83354 | East Equine Enceph Ab, IgG, S | 10896-9 |
83355 | East Equine Enceph Ab, IgM, S | 10898-5 |