Test Catalog

Test Id : BART

Bartonella Antibody Panel, IgG and IgM, Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis of Bartonella infection, especially in the context of a cat scratch

Highlights

This assay can be used as an aid to diagnose recent or past infection with Bartonella henselae or Bartonella quintana.

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Immunofluorescence Assay (IFA)

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Bartonella Ab Panel, IgG and IgM

Aliases
Lists additional common names for a test, as an aid in searching

Bartonella henselae Abs, IgG/IgM

Bartonella quintana, IgG/IgM

Bartonellosis

Cat Scratch Disease

BART

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Specimen Type
Describes the specimen type validated for testing

Serum

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.15 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
Frozen 30 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis of Bartonella infection, especially in the context of a cat scratch

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Bartonella henselae and Bartonella quintana are small, rod-shaped, pleomorphic, Gram-negative bacteria. The human body louse (Pediculus humanis) is the proposed vector for B quintana. No animal reservoir has been determined for B quintana. The domestic cat is believed to be both a reservoir and vector for B henselae. Cats may infect humans directly through scratches, bites, or licks; or indirectly through an arthropod vector. Humans remain the only host in which Bartonella infection leads to significant disease.

 

The sight of entry for Bartonella is through openings in the skin. Microscopically, Bartonella lesions appear as rounded aggregates that proliferate rapidly. These aggregates are masses of Bartonella bacteria. Warthin-Starry staining has shown that Bartonella organisms can be present within the vacuoles of endothelial cells, in macrophages, and between cells in areas of necrosis. Occasionally organisms are seen in the lumens of vessels. While cutaneous lesions are common, disseminated tissue infection by Bartonella has been seen in the blood, lymph nodes, spleen, liver, bone marrow, and heart. B henselae has been associated with cat scratch disease (CSD), peliosis hepatitis (PH), bacillary angiomatosis (BA), and endocarditis. B quintana has been associated with trench fever, BA, and endocarditis. BA is a vascular proliferative disease usually involving the skin and regional lymph nodes.

 

CSD begins as a cutaneous papule or pustule that usually develops within a week after an animal contact. Regional lymphadenopathy follows and is the predominant clinical feature of CSD. Trench fever, which was a significant problem during World War I and World War II, is characterized by a relapsing fever and severe pain in the shins. PH and febrile bacteremia syndrome are both syndromes that have afflicted patients with AIDS and patients who are immunocompromised. While trench fever and CSD are usually self-limiting illnesses, the other Bartonella-associated diseases can be life-threatening.

 

Interest in B quintana and B henselae has recently increased since its increased prevalence in patients with AIDS, in transplant patients, and those with suppressed immunity.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Bartonella henselae

IgG: <1:128

IgM: <1:20

 

Bartonella quintana

IgG: <1:128

IgM: <1:20

Interpretation
Provides information to assist in interpretation of the test results

A positive immunofluorescence assay (IFA) IgM (titer >1:20) suggests a current infection with either Bartonella henselae or Bartonella quintana.

 

A positive IgG (titer >1:128) suggests a current or previous infection. Increases in IgG titers in serial specimens suggest active infection.

 

Normal serum specimens usually have an IgG titer of less than 1:128. However, 5% to 10% of healthy controls exhibit a B henselae and B quintana titer of 1:128. Sera from healthy volunteers rarely show titers of 1:256 or greater. IgM titers in normal serum are typically less than 1:20. IgM titers at 1:20 or greater have not been seen in the normal population.

 

Molecular testing of tissue for Bartonella species nucleic acid is recommended in cases of suspected endocarditis.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Rodino KG, Stone E, Saleh OA, Theel ES: The Brief Case: Bartonella henselae endocarditis-a case of delayed diagnosis. J Clin Microbiol. 2019 Aug 26;57(9). e00114-19. doi: 10.1128/JCM.00114-19

2. Wolf LA, Cherry NA, Maggi RG, Breitschwerdt EB: In pursuit of a stealth pathogen: Laboratory diagnosis of bartonellosis. Clin Micro News. 2014;36(5):33-39

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

The Euroimmun indirect immunofluorescence test is a standardized in vitro assay for the determination of specific antibodies against Bartonella henselae and Bartonella quintana. BIOCHIP Mosaics are coated with B henselae and B quintana infected cells positioned next to each other in one reaction field. Samples are diluted and incubated on the substrate slides. If the reaction is positive, specific antibodies of class IgG and IgM attach to the antigens. In a second step, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and made visible using fluorescence microscopy. Semi quantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(Package insert: IIFT Bartonella henselae/Bartonella quintana (IgM) Instructions for the indirect immunofluorescence test. Euroimmun US, Inc; Version 7/18/2017)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Saturday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 3 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

86611 x 4

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
BART Bartonella Ab Panel, IgG and IgM 90251-0
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
15659 Bart Henselae IgG 6954-2
15660 Bart Henselae IgM 6955-9
15661 Bart Quintana IgG 44827-4
15662 Bart Quintana IgM 44825-8

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports