Evaluating patients with signs and symptoms compatible with a connective tissue disease, especially those patients with muscle pain and limb weakness, concomitant pulmonary signs and symptoms, Raynaud phenomenon, and arthritis
Testing for antibodies to Jo 1 is not useful in patients with a negative test for antinuclear antibodies
For more information see Connective Tissue Disease Cascade.
Multiplex Flow Immunoassay
Antibodies to Jo 1 Antigen
Autoantibodies to Jo 1 Antigen
Histidyl-tRNA Synthetase Antibodies
Jo 1 Antibodies
Polymyositis Antibodies
Jo 1 Antigen
For more information see Connective Tissue Disease Cascade.
Serum
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
0.35 mL
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | OK |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 21 days | |
Frozen | 21 days |
Evaluating patients with signs and symptoms compatible with a connective tissue disease, especially those patients with muscle pain and limb weakness, concomitant pulmonary signs and symptoms, Raynaud phenomenon, and arthritis
Testing for antibodies to Jo 1 is not useful in patients with a negative test for antinuclear antibodies
For more information see Connective Tissue Disease Cascade.
Jo 1 (histidyl tRNA synthetase) is a member of the amino acyl-tRNA
Jo 1 antibodies are a marker for the disease polymyositis, and occur
For more information see Connective Tissue Disease Cascade.
<1.0 U (negative
> or =1.0 U (positive
Reference values apply to all ages.
A positive result for Jo 1 antibodies is consistent with the diagnosis
A negative test for Jo 1 antibodies does not exclude the diagnosis of polymyositis or dermatomyositis.
1. Targoff I: Autoantibodies in polymyositis. Rheum Dis Clin North Am 1992;18:455
2. Leff R, Sherman J, Plotz P: Chapter 65: Inflammatory muscle diseases. In Clinical Immunology Principles and Practice, Second edition. Edited by R Rich, T Fleisher, W Shearer, B Kotzin, et al. St. Louis, Mosby-Year Book, 2001, pp 65.1-65.8
Recombinant Jo 1 antigen is coupled covalently to polystyrene microspheres, which are impregnated with fluorescent dyes to create a unique fluorescent signature. Jo 1 antibodies, if present in diluted serum, bind to the Jo 1 antigen on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated antihuman IgG antibody is then added to detect IgG anti-Jo 1 bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens, and a secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for Jo 1 microspheres to a 4-point calibration curve.(Package insert: Bioplex 2200 ANA Screen. Bio-Rad Laboratories, Hercules, CA 03/2012)
Monday through Saturday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
86235
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
JO1 | Jo 1 Ab, IgG, S | 33571-1 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
JO1 | Jo 1 Ab, IgG, S | 33571-1 |