Determining proliferation of tumor cells in paraffin-embedded tissue blocks from patients diagnosed with neuroendocrine tumors of the pancreas or gastrointestinal tract including metastases
Includes pathology consultation charged separately.
This is not an orderable test. Order PATHC / Pathology Consultation. The consultant will determine the need for special stains.
Immunohistochemistry, Semi- Quantitation, Hot-Spot Technique
Ki-67
MIB-1
Includes pathology consultation charged separately.
Special
This is not an orderable test. If ordering for diagnostic purposes, order PATHC / Pathology Consultation and request the stain.
Attach the green pathology address label included in the kit to the outside of the transport container.
1. Pathologist's name, address, and phone number are required.
2. Include accompanying pathology report stating the final diagnosis. If not available, a preliminary diagnosis is acceptable.
This is not an orderable test. Order PATHC / Pathology Consultation. The consultant will determine the need for special stains.
Supplies: Pathology Packaging Kit (T554
Specimen Type:
Preferred: Formalin-fixed, paraffin-embedded tissue block containing neuroendocrine tumor of the pancreas or gastrointestinal (GI) tract including metastases.
Acceptable: 2 unstained sections, containing neuroendocrine tumor of the pancreas or GI tract including metastases, on charged slides cut at 4 microns <1 month ago. Tissue on the slides should have been fixed in 10% neutral buffered formalin.
Submission Container/Tube: Pathology Packaging Kit (T554)
Collection Instructions: Submit formalin-fixed, paraffin-embedded tissue block
Additional Information: Paraffin block will be returned with the final report.
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Special | Ambient (preferred) | ||
Refrigerated |
Determining proliferation of tumor cells in paraffin-embedded tissue blocks from patients diagnosed with neuroendocrine tumors of the pancreas or gastrointestinal tract including metastases
Includes pathology consultation charged separately.
Ki-67(MIB-1 clone) is a monoclonal antibody that reacts with cells undergoing DNA synthesis by binding to the Ki-67 antigen, a marker known to be expressed only in proliferating cells. By measuring the amount of tumor cells expressing Ki-67, an estimate of DNA synthesis can be determined. Studies suggest that Ki-67(MIB-1) analysis of paraffin-embedded tissue specimens may provide useful prognostic information in various tumor types.
This is not an orderable test. Order PATHC / Pathology Consultation. The consultant will determine the need for special stains.
Varies by tumor type; values reported from 0% to 100%
Results will be reported as a percentage of tumor cells staining positive for Ki-67(MIB-1). Semi-quantitative Ki-67(MIB-1) results should be interpreted within the clinical context for which the test was ordered.
No significant cautionary statements
1. Bosman F, Carneiro F, Hruban R, et al: WHO classification of tumours of the digestive system. Lyon: International Agency for Research on Cancer, 2010
2. Hochwald SN, Zee S, Conlon KC, et al: Prognostic factors in pancreatic endocrine neoplasms: an analysis of 136 cases with a proposal for low-grade and intermediate-grade groups. J Clin Oncol 2002;20:2633-2642
3. Klimstra DS, Modlin IR, Coppola D, et al: The pathologic classification of neuroendocrine tumors: a review of nomenclature, grading, and staging systems. Pancreas 2009;39:707-712
4. Klimstra DS, Modlin IR, Adsay NV, et al: Pathology reporting of neuroendocrine tumors: application of the Delphic consensus process to the development of a minimum pathology data set. Am J Surg Pathol 2010;34:300-313
5. Pathology and Genetics Tumours of Endocrine Organs. Edited by RA DeLellis, RV Lloyd, PU Heitz, C Eng. IARC Press, 2004
A 4-micron thick section is cut from the paraffin block. The section is stained with an immunoperoxidase method using the monoclonal antibody Ki-67 (MIB-1 clone). This is the paraffin nuclear epitope to the Ki-67 antigen. Any nucleus that has an antigen-antibody complex will cause the bright-field, brown chromogen, diaminobenzidine (DAB), to precipitate onto it. All nuclei, both DAB-positive and -negative, are counterstained with diluted hematoxylin.(Unpublished Mayo method)
Monday through Friday
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
88360
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
KINM | Ki67 GI/Pancreas NET IHC Manual | 29593-1 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
71674 | Interpretation | 29593-1 |
71675 | Participated in the Interpretation | No LOINC Needed |
71676 | Report electronically signed by | 19139-5 |
71677 | Material Received | 81178-6 |
71678 | Disclaimer | 62364-5 |
71845 | Case Number | 80398-1 |