Test Catalog

Test Id : SUBIF

Immunoglobulin G (IgG) Subtypes Immunofluorescence, Tissue

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining the subclass of IgG antibody found in renal immunofluorescent panel and determining if the deposits are monoclonal or monotypic

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Direct Immunofluorescence

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

IGG Subtypes Immunofluorescence

Aliases
Lists additional common names for a test, as an aid in searching

RBIGG1IFR

RBIGG2IFR

RBIGG3IFR

RBIGG4IFR

Specimen Type
Describes the specimen type validated for testing

Special

Ordering Guidance

If additional interpretation/analysis is needed, request PATHC / Pathology Consultation along with this test and send the corresponding renal pathology light microscopy and immunofluorescence (IF) slides (or IF images on a CD), electron microscopy images (prints or CD), and the pathology report.

Shipping Instructions

1. Advise shipping specimens in Styrofoam transportation coolers to avoid extreme hot or cold temperatures to ensure specimens are received at required specimen stability temperature.

2. Attach the green pathology address label included in the kit to the outside of the transport container.

Necessary Information

A pathology/diagnostic report is required.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Preferred: Frozen tissue

Supplies: Renal Biopsy Kit (T231)

Specimen Type: Kidney tissue

Container/Tube: Renal Biopsy Kit, Zeus/Michel's, Frozen

Specimen Volume: Entire specimen

Collection Instructions: Collect specimens according to the instructions in Renal Biopsy Procedure for Handling Tissue for Light Microscopy (LM), Immunofluorescent Histology (IF), and Electron Microscopy (EM)

Additional Information: If standard immunoglobulin and complement immunofluorescence has already been performed, submit the residual frozen tissue (must contain glomeruli) on dry ice.

 

Acceptable: Frozen tissue

Slides: 4 frozen tissue unstained positively-charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

1. Renal Biopsy Patient Information

2. If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Special Frozen (preferred)
Ambient
Refrigerated

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining the subclass of IgG antibody found in renal immunofluorescent panel and determining if the deposits are monoclonal or monotypic

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

IgG subtypes are helpful in confirming some disease processes affecting the kidney.

Interpretation
Provides information to assist in interpretation of the test results

Staining intensity is graded as negative (0), weak (trace, 1+), moderate (2+) and strong (3+) and will be reported as such when not accompanied by a pathology consultation request.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Clinical Reference
Recommendations for in-depth reading of a clinical nature

Hemminger J, Nadasdy G, Satoskar A, Brodsky SV, Nadasdy T: IgG subclass staining in routine renal biopsy material. Am J Surg Pathol. 2016 May;40(5):617-626

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

Direct immunofluorescence staining on sections of fresh or frozen tissue.(Unpublished Mayo method)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 2 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

5 years

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

88346-primary IF

88350-if additional IF

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
SUBIF IGG Subtypes Immunofluorescence In Process
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
BA0271 Interpretation 50595-8
BA0272 Participated in the Interpretation No LOINC Needed
BA0276 Addendum 35265-8
BA0275 Report electronically signed by 19139-5
71618 Disclaimer 62364-5
71850 Case Number 80398-1
BA0274 Gross Description 22634-0
BA0273 Material Received 22633-2

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports