Marker of skeletal muscle differentiation
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IHTOI | IHC Initial, Tech Only | No | No |
IHTOA | IHC Additional, Tech Only | No | No |
Immunohistochemistry
ISMYOGEIHCTO
TECHONLY
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
1. Information on accessing digital images of immunohistochemical (IHC) stains and the manual requisition form can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/#FAQ
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Tissue/Other | Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
TECHONLY | Ambient (preferred) | ||
Refrigerated |
Marker of skeletal muscle differentiation
Myogenin is a member of a family of myogenic regulatory genes that include MyoD, myf5, and MRF4. These genes encode a set of transcription factors that are essential for muscle development. Expression of myogenin is restricted to cells showing skeletal muscle differentiation. Myogenin is found in the majority of rhabdomyosarcomas and Wilms tumors, and is absent in Ewing sarcoma and mature skeletal muscle.
This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.
The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.
1. Cessna MH, Zhou H, Perkins SL, et al: Are myogenin and myoD1 expression specific for rhabdomyosarcoma? Am J Surg Pathol 2001;25(9):1150-1157
2. Folpe AL: MyoD1 and myogenin expression in human neoplasia: A review and update. Adv in Anat Pathol 2002;9(3):198-203
3. Kumar S, Perlman E, Harris CA, et al: Myogenin is a specific marker for rhabdomyosarcoma: an immunohistochemical study in paraffin-embedded tissues. Mod Pathol 2000;13(9):988-993
4. Morgenstern DA, Rees H, Sebire NJ, et al: Rhabdomyosarcoma subtyping by immunohistochemical assessment of myogenin: tissue array study and review of the literature. Pathol and Oncol Res 2008;14(3):233-238
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
88342-TC, primary
88341-TC, if additional IHC
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
MYOGE | Myogenin IHC, Tech Only | Order only;no result |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
70828 | Myogenin IHC, Tech Only | Bill only; no result |