Phenotyping leukemias and lymphomas
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IHTOI | IHC Initial, Tech Only | No | No |
IHTOA | IHC Additional, Tech Only | No | No |
Immunohistochemistry
ISCD79IHCTO
TECHONLY
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Container/Tube: Immunostain Technical Only Envelope
Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
1. Information on accessing digital images of immunohistochemical (IHC) stains and the manual requisition form can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/#FAQ
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
TECHONLY | Ambient (preferred) | ||
Refrigerated |
Phenotyping leukemias and lymphomas
Cluster of differentiation 79a (CD79a) stains the cytoplasm and membrane of B lymphocytes and megakaryocytes. CD79a is a protein expressed on the surface of B lymphocytes at all stages of maturation, from B-lymphocyte precursors through plasma cells. Its function is to transduce the signal of antigen binding to immunoglobulin into the cytoplasm of the B lymphocyte initiating intracellular signaling. Antibodies to CD79a are diagnostically useful to demonstrate B-cell lineage of acute lymphoblastic leukemia, malignant lymphomas and chronic lymphoproliferative disorders.
This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.
1. Khurana S, Melody ME, Ketterling RP, et al: Molecular and phenotypic characterization of an early T-cell precursor acute lymphoblastic lymphoma harboring PICALM-MLLT10 fusion with aberrant expression of B-cell antigens. Cancer Genet. 2020;240:40-44. doi: 10.1016/j.cancergen.2019.11.002
2. Menter T, Lundberg P, Wenzel F, et al: RUNX1 mutations can lead to aberrant expression of CD79a and PAX5 in acute myelogenous leukemias: A potential diagnostic pitfall. Pathobiology. 2019;86(2-3):162-166. doi: 10.1159/000493688
3. Matnani RG, Stewart RL, Pulliam J, Jennings CD, Kesler M: Peripheral T-cell lymphoma with aberrant expression of CD19, CD20, and CD79a: Case report and literature review. Case Rep Hematol. 2013;2013:183134. doi: 10.1155/2013/183134
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
88342-TC, primary
88341-TC, if additional IHC
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
CD79 | CD79a IHC, Tech Only | Order only;no result |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
70731 | CD79a IHC, Tech Only | Bill only; no result |