A marker of immaturity in the setting of acute myeloid leukemia or B lymphoblastic leukemia
The diagnosis of gastrointestinal stromal tumors, solitary fibrous tumors, and angiosarcomas
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IHTOI | IHC Initial, Tech Only | No | No |
IHTOA | IHC Additional, Tech Only | No | No |
Immunohistochemistry
ISCD34IIHCTO
TECHONLY
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Container/Tube: Immunostain Technical Only Envelope
Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
1. Information on accessing digital images of immunohistochemical (IHC) stains and the manual requisition form can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/#FAQ
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Wet/frozen tissue; Cytology smears; Nonformalin fixed tissue; Nonparaffin embedded tissue; Noncharged slides; ProbeOn slides | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
TECHONLY | Ambient (preferred) | ||
Refrigerated |
A marker of immaturity in the setting of acute myeloid leukemia or B lymphoblastic leukemia
The diagnosis of gastrointestinal stromal tumors, solitary fibrous tumors, and angiosarcomas
CD34 is 115 kDa membrane associated antigen found on human hematopoietic progenitor cells and vascular endothelial cells. In normal tonsil sections, antibodies to CD34 strongly stain vascular endothelial cells.
CD34 is used as a marker of immaturity in the setting of acute myeloid leukemia or B lymphoblastic leukemia. It is also useful in the diagnosis of gastrointestinal stromal tumors, solitary fibrous tumors, and angiosarcomas.
This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.
1. Lopez-Nunez O, Surrey LF, Alaggio R, Fritchie KJ, John I: Novel PPP1CB-ALK fusion in spindle cell tumor defined by S100 and CD34 coexpression and distinctive stromal and perivascular hyalinization. Genes Chromosomes Cancer. 2020;59(8):495-499. doi: 10.1002/gcc.22844
2. Matoso A, Epstein JI: Epithelioid angiosarcoma of the bladder: A series of 9 cases. Am J Surg Pathol. 2015;39(10):1377-1382. doi: 10.1097/PAS.0000000000000444
3. Stanek J, Abdaljaleel M: CD34 immunostain increases the sensitivity of placental diagnosis of fetal vascular malperfusion in stillbirth. Placenta. 2019;77:30-38. doi: 10.1016/j.placenta.2019.02.001
4. Sardina LA, Piliang M, Bergfeld WF: Diagnostic value of CD34 and calretinin immunostaining in the diagnosis of proliferating tricholemmal tumor and trichoblastoma [published online ahead of print, 2019 Apr 23]. Int J Dermatol. 2019;10.1111/ijd.14461. doi: 10.1111/ijd.14461
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
88342-TC, primary
88341-TC, if additional IHC
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
CD34I | CD34 IHC, Tech Only | Order only;no result |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
70701 | CD34 IHC, Tech Only | Bill only; no result |