Test Id : CFCTB
Mycobacteria and Nocardia Culture, Cystic Fibrosis, Varies
Useful For
Suggests clinical disorders or settings where the test may be helpful
Detection and identification of nontuberculous Mycobacterium species from respiratory specimens of patients with cystic fibrosis
Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.
| Test Id | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ISMY | ID by 16S Sequencing | No, (Bill Only) | No |
| RMALM | Id MALDI-TOF Mass Spec AFB | No, (Bill Only) | No |
| RTBSP | Id, Mtb Speciation, PCR | No, (Bill Only) | No |
| TBT | Concentration, Mycobacteria | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| LCTB | Id, MTB complex Rapid PCR | No, (Bill Only) | No |
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, a reflex test may be performed at an additional charge.
Method Name
A short description of the method used to perform the test
Automated Detection of Positive Cultures followed by Organism Identification /DNA Sequencing/Matrix Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
Acid Fast Bacilli (AFB) Smear
Acid Fast Smear
Bacillus, acid-fast
Culture for TB (Tuberculosis)
Mycobacteria
Nocardia
Smear, Acid Fast Bacilli-AFB
TB (Tuberculosis) Culture
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, a reflex test may be performed at an additional charge.
Specimen Type
Describes the specimen type validated for testing
Varies
Ordering Guidance
If the patient does not have cystic fibrosis, chronic obstructive pulmonary disease, or bronchiectasis, routine mycobacterial culture should be ordered, see CTB / Mycobacteria and Nocardia Culture, Varies.
Necessary Information
1. Specimen source (anatomical body site) is required.
2. Alert the laboratory if Mycobacterium genavense is suspected, as this species requires addition of mycobactin J to the culture medium for optimal growth and recovery.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Respiratory fluid
Sources: Bronchoalveolar lavage fluid, bronchial washing, sputum (saliva is not acceptable)
Container/Tube: Sterile container
Specimen Volume: 3 to 5 mL
Collection Instructions:
1. Collect 3 separate respiratory specimens for acid-fast smears and culture in patients with clinical and chest X-ray findings compatible with tuberculosis.
2. These 3 specimens should be collected at 8 to 24-hour intervals (24 hours when possible) and should include at least 1 first-morning specimen.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
See Specimen Required
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Sources other than respiratory specimens, including blood, serum or fixed tissue | Reject |
| Environmental sources | Reject |
| Boric acid tubes | Reject |
| Saliva | Reject |
| Specimen in viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth) | Reject |
| Swabs (any type, source, or transport system) | Reject |
| Petri dish | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Ambient | 7 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Detection and identification of nontuberculous Mycobacterium species from respiratory specimens of patients with cystic fibrosis
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, a reflex test may be performed at an additional charge.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
This culture combines a traditional mycobacterial culture with an additional plate medium called NTM (nontuberculous mycobacteria) Elite agar, which enhances recovery of nontuberculous mycobacteria from respiratory specimens of patients with cystic fibrosis or other chronic pulmonary infections such as chronic obstructive pulmonary disease or bronchiectasis.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative
Interpretation
Provides information to assist in interpretation of the test results
A final negative report is issued after 42 days of incubation.
Positive cultures are reported as soon as detected.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Recovery of mycobacteria is dependent on the number of organisms present in the specimen, specimen collection methods, methods of processing, and patient factors such as use of anti-mycobacteria therapy.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Martin I, Pfyffer GE, Parrish N: Mycobacterium: General characteristics, laboratory detection and staining procedures. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2011:472-502
2. Caldwell M, Tisdale J, Khare R. Improved recovery of nontuberculous mycobacteria in culture with adjunctive use of a selective agar. J Clin Microbiol. 2024;62(3):e0167823. doi:10.1128/jcm.01678-23
3. Broncano-Lavado A, Barrado L, Lopez-Roa P, et al. Clinical evaluation of nontuberculous mycobacteria (NTM) elite agar, a new medium for the isolation of NTM: a multicenter study. J Clin Microbiol. 2023;61(4):e0003623. doi:10.1128/jcm.00036-23
Method Description
Describes how the test is performed and provides a method-specific reference
The BACTEC MGIT 960 System is a broth system designed for the rapid detection of mycobacteria in clinical specimens. Mycobacteria growth indicator tubes (MGITs) are incubated for up to 42 days and growth is evaluated with mycobacteria identified as soon as an MGIT signals positive on the instrument.
In addition to the MGIT tube, Middlebrook 7H10/7H10S agar biplates and an NTM Elite agar plate is inoculated and incubated at 37 degrees C for 42 days. Growth from positive MGITs or agar plates is identified using a variety of techniques as appropriate including rapid polymerase chain reaction, matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, or 500 base pair 16S rRNA gene sequencing.(Plongla R, Preece CL, Perry JD, Gilligan PH. Evaluation of RGM medium for isolation of nontuberculous mycobacteria from respiratory samples from patients with cystic fibrosis in the United States. J Clin Microbiol. 2017;55[5]:1469-1477. doi:10.1128/JCM.02423-16; Stephenson D, Perry A, Appleby MR, et al. An evaluation of methods for the isolation of nontuberculous mycobacteria from patients with cystic fibrosis, bronchiectasis and patients assessed for lung transplantation. BMC Pulm Med. 2019;19[1]:19. Published 2019 Jan 21. doi:10.1186/s12890-019-0781-2)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Sunday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
87116-Mycobacterial Culture
87015-Mycobacteria Culture, Concentration (if appropriate)
87118-Id MALDI-TOF Mass Spec AFB (if appropriate)
87150-Id, Mtb Speciation, PCR (if appropriate)
87153-Mycobacteria Identification by Sequencing (if appropriate)
87176-Tissue Processing (if appropriate)
87150- Id, MTB complex Rapid PCR (if appropriate)
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| CFCTB | Mycobacterial Culture, Cystic Fibro | 543-9 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| CFCTB | Mycobacterial Culture, Cystic Fibro | 543-9 |