Test Id : BTP
Beta-Trace Protein, Body Fluid
Useful For
Suggests clinical disorders or settings where the test may be helpful
Determining the presence of cerebrospinal fluid in body fluids
Method Name
A short description of the method used to perform the test
Nephelometry
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
B2 Transferrin
Beta-2 Transferrin
Cerebrospinal Fluid Leakage
CSF Specific Transferrin
Otorrhea, Spinal Fluid
Prostaglandin D2 synthase
Rhinorrhea, Spinal Fluid
Tau Protein
Transferrin B2
Transferrin, Spinal Fluid
Specimen Type
Describes the specimen type validated for testing
Body Fluid
Additional Testing Requirements
If specimens are collected from multiple sites on the body (ie, left and right), each specimen must be sent under a separate order.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Body fluid
Collection Container/Tube:
Preferred: Sterile container, syringe (with needle removed), test tube, or microtube
Acceptable: Plain cotton swab, pledget, gauze, or facial tissue
Specimen Volume: 0.5 mL
Collection Instructions:
1. If submitting a syringe, remove the needle. Add cap to end of syringe.
2. If direct collection is not feasible, specimen may be collected using a plain cotton swab, pledget, gauze or facial tissue.
a. For gauze or facial tissue: circle area on the gauze where specimen was collected.
b. For swab, pledget, gauze or facial tissue: place in a small container (plain test tube or sterile container).
3. Do not collect specimen with a culture swab.
4. Do not add any additional liquid other than source to the swab or gauze.
5. Do not collect or send swab or gauze specimens in containers with additional liquids or additives.
Additional Information:
1. Although results may be obtainable on smaller specimens (perhaps as little as 0.05 mL, depending on the protein concentrations and percentage of spinal fluid in the specimen), reliable results are best obtained with an adequate specimen volume.
2.Specimens collected with additives such as microbiology media (eg, Stuart or Amies liquid medium) or TransFix/EDTA (used for analyses in flow cytometry) yield uninterpretable results and will be rejected.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
See Specimen Required
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
| Specimens with additives (eg, microbiology transport media, TransFix/EDTA) | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Body Fluid | Refrigerated (preferred) | 14 days | |
| Ambient | 7 days | ||
| Frozen | 30 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Determining the presence of cerebrospinal fluid in body fluids
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
The diagnosis of cerebrospinal fluid (CSF) rhinorrhea or otorrhea (leakage of CSF into the nose or ear canal, usually as a result of head trauma, tumor, congenital malformation, or surgery) is often difficult to confirm. Traditional chemical analyses (eg, glucose, protein, specific gravity) are unreliable. Radiographic studies, especially those involving the injection of dyes or radiographic compounds, are costly and may introduce additional risks to the patient. Prompt diagnosis and localization facilitates appropriate decisions and decreases the risk of meningitis.
Beta-trace protein (prostaglandin D synthase) is one of the most abundant proteins in CSF and has a very low concentration in other body fluids. Elevated concentrations of beta-trace protein in body fluid or drainage are consistent with CSF leakage.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Beta-trace protein concentration <5 mg/L is negative for cerebrospinal fluid
Beta-trace protein concentration 5-7 mg/L is indeterminate for presence of cerebrospinal fluid
Beta-trace protein concentration >7 mg/L is consistent with the presence of cerebrospinal fluid
Interpretation
Provides information to assist in interpretation of the test results
Beta-trace protein is produced within the cerebrospinal fluid (CSF) and typically has a greater than 10-fold higher concentration in the CSF versus blood plasma.
Beta-trace protein concentrations above 7 mg/L are 84% sensitive and 97% specific for the presence of CSF.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Interferences by rheumatoid factors are generally suppressed by the use of the N BTP (beta trace protein) Supplementary Reagent.
Low volume of cerebrospinal fluid specimens may reduce the beta trace protein recovery.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Mantur M, Lukaszewicz-Zajac M, Mroczko B, et al. Cerebrospinal fluid leakage-reliable diagnostic methods. Clin Chim Acta. 2011;412(11-12):837-840. doi:10.1016/j.cca.2011.02.017
2. Risch L, Lisec I, Jutzi M, Podvinec M, Landolt H, Huber AR. Rapid, accurate and non-invasive detection of cerebrospinal fluid leakage using combined determination of B-trace protein in secretion and serum. Clin Chim Acta. 2005;351(1-2):169-176. doi:10.1016/j.cccn.2004.09.008
3. Bernasconi L, Potzl T, Steuer C, Dellweg A, Metternich F, Huber AR. Retrospective validation of a B-trace protein interpretation algorithm for the diagnosis of cerebrospinal fluid leakage. Clin Chem Lab Med. 2017;55(4):554-560. doi:10.1515/cclm-2016-0442
4. Phang SY, Whitehouse K, Lee L, Khalil H, McArdle P, Whitfield PC. Management of CSF leak in base of skull fractures in adults. Br J Neurosurg. 2016;30(6):596-604. doi:10.1080/02688697.2016.1229746
5. Lipschitz N, Hazenfield JM, Breen JT, Samy RN. Laboratory testing and imaging in the evaluation of cranial cerebrospinal fluid leaks and encephaloceles. Curr Opin Otolaryngol Head Neck Surg. 2019;27(5):339-343. doi:10.1097/MOO.0000000000000578
Method Description
Describes how the test is performed and provides a method-specific reference
Polystyrene particles coated with antibodies to human B-trace protein are agglutinated when mixed with samples containing B-trace protein. The intensity of the scattered light in the nephelometer depends on the concentration of the analyte in the sample and consequently its concentration can be determined by comparison with dilutions of a standard of known concentration.(Package insert: N Latex BTP. Siemens; Rev 06. 10/2022)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
83883
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| BTP | Beta-Trace Protein, BF | 57733-8 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| BTPC | Beta-Trace Protein, BF | 57733-8 |