Test Id : LCYP
Cytokine Panel, 13 Analytes, Quantitative, Serum
Useful For
Suggests clinical disorders or settings where the test may be helpful
Aiding in the assessment of the pathophysiology of immune, infectious, or inflammatory disorders when used in conjunction with standard clinical assessment
May be used for research purposes
Method Name
A short description of the method used to perform the test
Immunoassay
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
Cytokine 12
Cytokine
Cytokines
IL2 Receptor
IL2R
IL4
IL5
IL6
IL 2R
IL 4
IL 5
IL 6
IL 8
IL2 R
IL17
IL 1B
IL 10
IL 12
IL 13
IL-12
IL-13
IL-2
IL-2R
Il 1
IL-5
IL-6
IL-8
IL1
IL1 B
IL1 Beta
IL2
IFN
IL 1 B
IL 2
IL 2 R
IFN Gamma
IL
IL 17
IFN G
IL-1 Beta
IL-4
IL10
IL12
IL13
IL8
TNF Alpha
Tumor Necrosis Factor alpha
IL 0
Specimen Type
Describes the specimen type validated for testing
Serum
Shipping Instructions
Specimen must be shipped frozen. Testing will be canceled if received ambient or refrigerated.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Collection Container/Tube: Serum gel or red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL Serum
Collection Instructions:
1. As soon as possible or within 2 hours of collection, centrifuge and aliquot 1 mL of serum into a plastic vial.
2. Send frozen.
Note: Critical frozen. Separate specimens must be submitted when multiple tests are ordered.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
Serum: 0.5 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Heat-inactivated specimens | Reject |
| Contaminated specimens | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen | 30 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Aiding in the assessment of the pathophysiology of immune, infectious, or inflammatory disorders when used in conjunction with standard clinical assessment
May be used for research purposes
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Cytokines play a pivotal role in both innate and adaptive immune pathways by mediating diverse cellular functions. Elevated cytokines have been reported in infection, autoimmune diseases, malignancy, post-transplant states, immune deficiency with dysregulation, and after immunomodulatory therapy. Cytokine levels have demonstrated utility as biomarkers in autoinflammatory and autoimmune diseases and for monitoring treatment responses to biologics. Multiplex cytokine profiling can reveal characteristic patterns, including broad elevation of pro-inflammatory mediators (such as interleukin [IL]-6, tumor necrosis factor alpha [TNF-alpha], IL-8) in systemic inflammation, increased Th1-type cytokines (such as IFN-gamma, IL-2) in cell-mediated activation, and elevated Th2-type cytokines (such as IL-4, IL-5, IL-13) in allergic or certain antibody-mediated processes, while increased IL-10 or soluble IL-2 receptor may indicate regulatory activation or lymphoproliferation. Cytokine serum panels can help distinguish IL-1beta-driven autoinflammatory syndromes (eg, familial Mediterranean fever) from conditions more strongly associated with TNF-alpha and IL-6 (eg, rheumatoid arthritis). Elevated levels of soluble IL-2R and IL-10, often accompanied by increased interferon gamma (IFN-gamma) and IL-6, have been shown to differentiate complicated common variable immunodeficiency (CVID) patients with autoimmunity and/or lymphoproliferation from those with predominantly infection-only phenotypes. The cytokine storm (CS), observed in sepsis, CAR-T cell therapy, COVID-19 infection and other infectious and noninfectious conditions, is characterized by markedly increased circulating cytokine levels due to overactivation of immune cells. Measuring cytokine levels following CAR-T cell therapy is important for characterizing therapy-induced toxicities such as cytokine release syndrome (CRS). Elevations in cytokines including IL-1beta, IL-2, IL-6, IL-8, IL-10, IFN-gamma, and TNF-alpha have been reported in CRS, with higher and earlier peaks within the first week after infusion generally correlating with greater severity. Characterization of CS and CRS facilitates earlier and more targeted intervention with available cytokine-directed treatments (such as antibodies targeting IL-1, IL-6, TNF-alpha, IFN-gamma) in appropriate clinical contexts, to improve outcomes and reduce the risk of life-threatening complications.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Tumor Necrosis Factor-alpha: < or =15.4 pg/mL
Interleukin 2: < or =1.3 pg/mL
Interleukin 2 Receptor Soluble: 353.0-1745 pg/mL
Interleukin 12: < or =1.8 pg/mL
Interferon gamma: < or =3.7 pg/mL
Interleukin 4: < or =1.0 pg/mL
Interleukin 5: < or =2.5 pg/mL
Interleukin 10: < or =2.9 pg/mL
Interleukin 13: < or =21.0 pg/mL
Interleukin 17: < or =1.3 pg/mL
Interleukin 1 beta: < or =5.7 pg/mL
Interleukin 6: < or =5.3 pg/mL
Interleukin 8: < or =20.7 pg/mL
Interpretation
Provides information to assist in interpretation of the test results
Results are used to understand the pathophysiology of immune, infectious, or inflammatory disorders, or may be used for research purposes.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Cytokine levels may demonstrate diurnal variation. For longitudinal comparison, it is recommended that cytokine levels be determined at the same time of day.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Chetaille Nezondet AL, Poubelle PE, Pelletier M. The evaluation of cytokines to help establish diagnosis and guide treatment of autoinflammatory and autoimmune diseases. J Leukoc Biol. 2020;108(2):647-657. doi:10.1002/JLB.5MR0120-218RRR
2. Liu C, Chu D, Kalantar-Zadeh K, George J, et al. Cytokines: From clinical significance to quantification. Adv Sci (Weinh). 2021;8(15):e2004433. doi:10.1002/advs.202004433
3. Jogdand A, Gilbert KM, Hong JS, et al. Utility of serum cytokine testing to differentiate complicated common variable immunodeficiency in resource limited settings. J Allergy Clin Immunol Glob. 2025;4(3):100488. Published 2025 Apr 23. doi:10.1016/j.jacig.2025.100488
4. Nie J, Zhou L, Tian W, et al. Deep insight into cytokine storm: from pathogenesis to treatment. Signal Transduct Target Ther. 2025;10(1):112. Published 2025 Apr 16. doi:10.1038/s41392-025-02178-y5. Hughes AD, Teachey DT, Diorio C. Riding the storm: managing cytokine-related toxicities in CAR-T cell therapy. Semin Immunopathol. 2024;46(3-4):5. Published 2024 Jul 16. doi:10.1007/s00281-024-01013-w
Method Description
Describes how the test is performed and provides a method-specific reference
Analyte specific antibodies are pre-coated onto a microfluidic Simple Plex cartridge. Samples are diluted and added to the cartridge. The sample runs through a microfluidic channel that binds the protein of interest. The Ella platform washes off any unbound analyte and adds a detection reagent. Each channel utilized for analyte capture encompasses three glass nano reactors coated with a capture antibody, providing analyte values in triplicate. A calculated analyte value is then generated from the factory-calibrated standard curve that is built into every cartridge.
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Tuesday, Thursday, Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test was developed and its performance characteristics determined by Progentec Diagnostics. It has not been cleared by the US Food and Drug Administration (FDA). This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
83520 x 12
83529
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| LCYP | Cytokine Panel, Quantitative, S | Not Provided |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| CYP01 | Tumor Necrosis Factor alpha (TNF-a) | Not Provided |
| CYP02 | Interleukin 2 (IL-2) | Not Provided |
| CYP03 | Soluble Interleukin-2 Recep(sIL-2R) | Not Provided |
| CYP04 | Interleukin 12 (IL-12) | Not Provided |
| CYP05 | Interferon gamma (IFN-g) | Not Provided |
| CYP06 | Interleukin 4 (IL-4) | Not Provided |
| CYP07 | Interleukin 5 (IL-5) | Not Provided |
| CYP08 | Interleukin 10 (IL-10) | Not Provided |
| CYP09 | Interleukin 13 (IL-13) | Not Provided |
| CYP10 | Interleukin 17 (IL-17) | Not Provided |
| CYP11 | Interleukin 1 beta (IL-1b) | Not Provided |
| CYP12 | Interleukin 6 (IL-6) | Not Provided |
| CYP13 | Interleukin 8 (IL-8) | Not Provided |