Test Id : DMAID
Mycobacterium abscessus Complex Subspecies Identification, Next-Generation Sequencing
Useful For
Suggests clinical disorders or settings where the test may be helpful
Determining the subspecies of a Mycobacterium abscessus complex culture isolate
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, subspecies identification will always be performed at an additional charge if the organism is not already identified to the subspecies level.
Method Name
A short description of the method used to perform the test
Next-Generation Sequencing (NGS)
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
Next Generation Sequencing Test
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, subspecies identification will always be performed at an additional charge if the organism is not already identified to the subspecies level.
Specimen Type
Describes the specimen type validated for testing
Varies
Ordering Guidance
This test is used to identify the subspecies of Mycobacterium abscessus from a known M abscessus isolate.
For the identification of M abscessus (not the subspecies) from culture isolate growth, order CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Necessary Information
Specimen source and suspected organism identification are required.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Supplies: Infectious Container, Large (T146)
Specimen Type: Mycobacterium abscessus isolate growing in pure culture
Container/Tube:
Preferred: Solid slant medium (eg. Middlebrook 7H10, 7H11 or Lowenstein Jensen agar)
Acceptable: Broth medium (eg. Mycobacteria Growth Indicator Tube, 7H9 broth, BACT/ALERT MP or VersaTREK bottle). Note: Broth specimens will require subculture which will delay results.
Specimen Volume:
Solid slant medium: Isolate with visible growth
Broth medium: Greater than or equal to 3 mL of broth culture
Collection Instructions:
1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
See Specimen Required
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Agar plate | Reject |
| Mixed culture | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Useful For
Suggests clinical disorders or settings where the test may be helpful
Determining the subspecies of a Mycobacterium abscessus complex culture isolate
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, subspecies identification will always be performed at an additional charge if the organism is not already identified to the subspecies level.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
This assay provides a subspecies-level identification of microbiologic culture isolates previously identified to be Mycobacterium abscessus. Subspecies level identification can be important for patient care and for epidemiologic investigations since the subspecies can differ in macrolide susceptibility.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Not applicable
Interpretation
Provides information to assist in interpretation of the test results
This assay can differentiate the subspecies of Mycobacterium abscessus, which are, M abscessus subspecies abscessus, M abscessus subspecies bolletii, and M abscessus subspecies massiliense.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Only pure culture isolates of Mycobacterium abscessus should be submitted. Mixed cultures will result in a delay because the M abscessus must be isolated prior to performing the next-generation sequencing assay.
This assay has not been verified for the direct detection and direct subspecies identification of M abscessus from clinical specimens. It is intended for use on microbiologic culture isolates already identified as M abscessus.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1 Daley CL, Iaccarino JM, Lange C, et al. Treatment of Nontuberculous Mycobacterial Pulmonary Disease: An Official ATS/ERS/ESCMID/IDSA Clinical Practice Guideline. Clin Infect Dis. 2020;71(4):905-913. doi:10.1093/cid/ciaa1125
2. Kumar K, Daley CL, Griffith DE, Loebinger MR. Management of Mycobacterium avium complex and Mycobacterium abscessus pulmonary disease: therapeutic advances and emerging treatments. Eur Respir Rev. 2022;31(163):210212. Published 2022 Feb 9. doi:10.1183/16000617.0212-2021
Method Description
Describes how the test is performed and provides a method-specific reference
The subspecies of Mycobacterium abscessus are differentiated using the Genoscreen Deeplex Myc-TB assay. This test is a targeted next generation sequencing test for identification of Mycobacterium species using deep sequencing of a single 24-plexed amplicon mix for identification. The gene target used in this assay for species identification is primarily hsp65 but additional targets such as rrl, rrs, rpoB are used to help with nontuberculous mycobacteria identification to permit discrimination of closely related species and subspecies. This test was validated to demonstrate its ability to differentiate the 3 subspecies within the M abscessus from microbiologic culture isolates using the Nextera XT DNA Library Preparation kit, the MiSeq Reagent Kit V2. 300 cycle kit and the Illumina MiSeq sequencing platform.(Buckwalter SP, Olson SL, Fida M, et al. Mycobacterium abscessus subspecies identification using the Deeplex Myc-TB targeted NGS assay. J Clin Microbiol. 2023;61(10):e0048923. doi:10.1128/jcm.00489-23)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
87153
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| DMAID | M abscessus subspecies ID, NGS | 112278-7 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| DMAID | M abscessus subspecies ID, NGS | 112278-7 |