Identification of colorectal carcinoma and rectal neuroendocrine tumors
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IHTOI | IHC Initial, Tech Only | No | No |
IHTOA | IHC Additional, Tech Only | No | No |
Immunohistochemistry (IHC)
TECHONLY
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
1. Information on accessing digital images of immunohistochemical (IHC) stains and the manual requisition form can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/#FAQ
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
TECHONLY | Ambient (preferred) | ||
Refrigerated |
Identification of colorectal carcinoma and rectal neuroendocrine tumors
Special AT-rich sequence binding protein 2 (SATB2) is a transcription factor that regulates gene expression through chromatin remodeling. SATB2, when used in combination with the marker Keratin 20, may identify more than 95% of colorectal carcinoma. It may also be used to differentiate rectal neuroendocrine tumors from other neuroendocrine tumors of the gastrointestinal tract.
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; contact 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.
1. Berg KB, Schaeffer DF: SATB2 as an Immunohistochemical Marker for Colorectal Adenocarcinoma, A concise Review of Benefits and Pitfalls. Arch Pathol Lab Med 2017;141(10):1428-1433
2. Davis JL, Horvai AE: Special AT-rich sequence-binding protein 2 (SATB2) expression is sensitive but may not be specific for osteosarcoma as compared with other high-grade primary bone sarcomas. Histopathology 2016;69(1):84-90
3. Dragomir A, de Wit M, Johansson C, et al: The role of SATB2 as a diagnostic marker for tumors of colorectal origin. Am J Clin Pathol 2014;141(5):630-638
4. Magnusson K, de Wit M, Brennan DJ, et al: SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinoma. Am J Surg Pathol 2011;35(7):937-948
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
88342-TC, primary
88341-TC, if additional IHC
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
SATB2 | SATB2 IHC, Tech Only | Order only;no result |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
607601 | SATB2 IHC, Tech Only | Bill only; no result |