Test Catalog

Test Id : YERSC

Yersinia Culture, Feces

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining whether Yersinia species may be the cause of diarrhea

 

Reflexive testing for Yersinia species from nucleic acid amplification test-positive feces

 

This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.

Highlights

This test provides evidence of the presence of the bacterium, Yersinia species, in feces, in a viable state, and provides an isolate for antibacterial susceptibility testing and, if needed, submission to a health department.

 

Minnesota healthcare providers are required to report all confirmed or suspected cases of enteric yersiniosis to the Minnesota Department of Health. Mayo Clinic Laboratories clients should refer to their local health departments regarding public health submission of Yersinia isolates.

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged separately.

See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Conventional Culture

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Yersinia Culture, F

Aliases
Lists additional common names for a test, as an aid in searching

Aerobic Bacterial Stool Culture

Enteric pathogens

Yersinia Stool Culture

Yersinia

Stool Culture

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged separately.

See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Specimen Type
Describes the specimen type validated for testing

Fecal

Ordering Guidance

This test is not intended to diagnose plague, which is caused by Yersinia pestis. Contact the laboratory for cases of suspected plague.

Additional Testing Requirements

If susceptibility testing is needed, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Yersinia species from feces), it will not be performed and the ZMMLS order will be canceled at time of report.

 

In some cases, local public health requirements may impact Mayo Clinic Laboratories clients, requiring, for example, submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Yersinia species recovered from specimens submitted to Mayo Clinic Laboratories by calling 800-533-1710 as soon as possible after reporting (to ensure viability of the bacterium).

Shipping Instructions

Specimen must arrive within 96 hours of collection.

Necessary Information

Specimen source is required.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
Q00M0086 Specimen Source

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Patient Preparation: Medications: Do not use barium or bismuth before collection of specimen.

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Specimen Type: Preserved Feces

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Unpreserved feces ECOFIX preservative Formalin, or PVA fixative Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 4 days
Refrigerated 4 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining whether Yersinia species may be the cause of diarrhea

 

Reflexive testing for Yersinia species from nucleic acid amplification test-positive feces

 

This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged separately.

See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Diarrhea may be caused by a number of agents, including bacteria, viruses, parasites, and chemicals; these agents may result in similar symptoms. A thorough patient history covering symptoms, severity and duration of illness, age, travel history, food consumption, history of recent antibiotic use, and illnesses in the family or other contacts will help the healthcare provider determine the appropriate testing to be performed.

 

Several species of Yersinia that are detected by this test may cause diarrhea. Yersinia enterocolitica is the species most commonly isolated with this test.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

No growth of pathogen

Interpretation
Provides information to assist in interpretation of the test results

The growth of Yersinia species identifies a potential cause of diarrhea.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

The yield of Yersinia species is reduced when specimens are delayed in transit to the laboratory (>2 hours from collection for unpreserved specimens).

 

Check local public health requirements, which may require submission of isolates to a public health laboratory. Not all species of Yersinia are detected by this test.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Pillai DR: Fecal culture for aerobic pathogens of gastroenteritis. In Clinical Microbiology Procedures Handbook, Fourth edition. Washington, DC, ASM Press, 2016, Section 3.8.1

2. Pillai DR: Fecal culture for Campylobacter and related organisms. In Clinical Microbiology Procedures Handbook, Fourth edition. Washington, DC, ASM Press, 2016, Section 3.8.2

3. DuPont HL: Persistent diarrhea: A clinical review. JAMA 2016;315(24):2712-2723 doi:10.1001/jama.2016.7833

4. Tacket CO, Narain JP, Sattin R, et al: A multistate outbreak of infections caused by Yersinia enterocolitica transmitted by pasteurized milk. JAMA 1984;251:483

5. El Qouqa IA, El Jarou MA, Samaha AS, et al. Yersinia enterocolitica infection among children aged less than 12 years: a case-control study. Int J Infect Dis 2011;15:e48

6. Loftus CG, Harewood GC, Cockerill FR 3rd, Murray JA: Clinical features of patients with novel Yersinia species. Dig Dis Sci 2002;47:2805

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

The fecal specimen is inoculated onto cefsulodin-irgasan-novobiocin (CIN) agar and phosphate buffered saline (PBS) for cold enrichment. After incubation, suspect Yersinia colonies are identified using one or a combination of the following techniques: Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, conventional biochemical tests, carbon source utilization, serologic methods, or nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene. Yersinia species that may be reported include but are not limited to: Y enterocolitica; Y frederiksenii; Y intermedia; Y kristensenii; Y pestis; Y pseudotuberculosis; and Y similis.(Kingry LC, Tarr CL, Petersen JM: Chapter 41: Yersinia. In Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller. Washington DC,ASM Press, 2019, pp 751-764)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

24 to 27 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87046-Yersinia Culture, Stool-with isolation and preliminary examination

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
YERSC Yersinia Culture, F 28549-4
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
YERSC Yersinia Culture, F 28549-4

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports