Aids in the identification of high grade neuroendocrine carcinomas and small cell carcinomas
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IHTOI | IHC Initial, Tech Only | No | No |
IHTOA | IHC Additional, Tech Only | No | No |
Immunohistochemistry
ISRBIHCTO
TECHONLY
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Container/Tube: Immunostain Technical Only Envelope (T693)
Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
1. Information on accessing digital images of immunohistochemical (IHC) stains and the manual requisition form can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/#FAQ
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Tissue/Other | Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
TECHONLY | Ambient (preferred) | ||
Refrigerated |
Aids in the identification of high grade neuroendocrine carcinomas and small cell carcinomas
Retinoblastoma (Rb) is a phosphoprotein that is expressed in most normal cells and acts as a tumor suppressor by providing a cell cycle checkpoint between G1 and S phases. Loss of nuclear expression of Rb is useful in the identification of neuroendocrine carcinomas and small cell carcinomas. Loss of Rb can also be helpful to differentiate spindle cell lipomas from myofibroblastomas and cellular angiofibromas from other genital stromal lesions.
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.
2. Yuan J, Knorr J, Altmannsberger M, et al: Expression of p16 and Lack of pRB in Primary Small Cell Lung Cancer. J Pathol 1999;189:358-362
3. Chen B, Marino-Enriquez A, Fletcher C, et al: Loss of Retinoblastoma Protein Expression in Spindle Cell/Pleomorphic Lipomas and Cytogenetically Related Tumors: An Immunohistochemical Study With Diagnostic Implications. Am J Surg Pathol 2012;36(8):1119-1128
4. Tang L, Basturk O, Sue J, et al: A Practical Approach to the Classification of WHO Grade 3 (G3) Well-differentiated Neuroendocrine Tumor (WD-NET) and Poorly Differentiated Neruoendocrine Carcinoma (PD-NEC) of the Pancreas. Am J Surg Pathol 2016;40(9):1192-1202
5. Konukiewitz B, Schlitter A, Jesinghaus M, et al: Somatostatin receptor expression related to TP53 and RB1 alterations in pancreatic and extrapancreatic neuroendocrine neoplasms with a Ki67-index above 20%. Mod Pathol 2017;1-12
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
88342-TC, primary
88341-TC, if additional IHC
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
RB1 | Retinoblastoma IHC, Tech Only | Order only;no result |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
604029 | Retinoblastoma IHC, Tech Only | Bill only; no result |