Test Catalog

Test Id : LASF1

Lactic Acid, Spinal Fluid

Useful For
Suggests clinical disorders or settings where the test may be helpful

Aid in differentiating between bacterial and viral meningitis

 

Aid in identifying increased anaerobic glycolysis or hypoxia associated with bacterial meningitis, cerebral infarction, cerebral arteriosclerosis, intracranial hemorrhage, hydrocephalus, traumatic brain injury, cerebral edema, epilepsy, and inborn errors of metabolism

Method Name
A short description of the method used to perform the test

Colorimetric

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Lactic Acid, CSF

Aliases
Lists additional common names for a test, as an aid in searching

L-Lactate

Lactate

Lactate (L-Lactate)

Lactic Acid (L-Lactate)

Specimen Type
Describes the specimen type validated for testing

CSF

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Specimen Type: Spinal fluid

Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions: Centrifuge to remove any cellular material.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis OK
Gross lipemia OK

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
CSF Frozen (preferred) 60 days
Refrigerated 24 hours
Ambient 3 hours

Useful For
Suggests clinical disorders or settings where the test may be helpful

Aid in differentiating between bacterial and viral meningitis

 

Aid in identifying increased anaerobic glycolysis or hypoxia associated with bacterial meningitis, cerebral infarction, cerebral arteriosclerosis, intracranial hemorrhage, hydrocephalus, traumatic brain injury, cerebral edema, epilepsy, and inborn errors of metabolism

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Anaerobic glycolysis markedly increases lactate concentrations. Lactate concentrations in cerebrospinal fluid (CSF) are increased in the presence of cerebral glycolysis or hypoxia associated with bacterial meningitis, cerebral infarction, cerebral arteriosclerosis, intracranial hemorrhage, hydrocephalus, traumatic brain injury, cerebral edema, epilepsy, and inborn errors of metabolism. Lactate found in CSF is predominantly produced by central nervous system (CNS) anaerobic glycolysis and is independent of blood lactate. Lactate measurement in CSF has been proposed as a test to differentiate bacterial from viral meningitis.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

0-2 days: 1.1-6.7 mmol/L

3-10 days: 1.1-4.4 mmol/L

11 days-17 years: 1.1-2.8 mmol/L

>17 years: 1.1-2.4 mmol/L

Interpretation
Provides information to assist in interpretation of the test results

In addition to reference intervals, published meta-analysis of 33 studies concluded concentrations greater than 3.9 mmol/L are suggestive of bacterial meningitis, with lower concentrations suggestive of viral meningitis.(1)

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Cerebrospinal fluid (CSF) lactate concentrations should be interpreted in conjunction with clinical findings and other laboratory results.

 

CSF lactate concentrations decrease after treatment with antibiotics; therefore, specimens should be collected prior to initiation of antibiotics in order to differentiate bacterial from aseptic meningitis.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Sakushima K, Hayashino Y, Kawaguchi T, et al: Diagnostic accuracy of cerebrospinal fluid lactate for differentiating bacterial meningitis from aseptic meningitis: A meta-analysis. J Infect 2011;62:255-262

2. Zhang W, Natowicz MR: Cerebrospinal fluid lactate and pyruvate concentrations and their ratio. Clin Biochem 2013;46:694-697

Method Description
Describes how the test is performed and provides a method-specific reference

Lactate concentration is determined using an enzymatic colorimetric method. L-lactate is oxidized to pyruvate by the specific enzyme lactate oxidase. Peroxidase is used to generate a colored dye using the hydrogen peroxide generated in the first reaction. The intensity of the color formed is directly proportional to the L-lactate concentration. It is determined by measuring the increase in absorbance.(Package insert: Roche Diagnostics Cobas 6000; LACT2 reagent package insert; Indianapolis, IN 46256. 02/2016)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 2 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

83605

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports