Limulus Amebocyte Lysate (LAL) Pathway
Beta D Glucan
Specimen Type: Spinal Fluid
Container/Tube: Sterile container
Specimen Volume: 2 mL
Collection Instructions: Collect 2 mL of spinal fluid (CSF) in a sterile container. Ship frozen.
|Hemolysis||Mild reject; Gross reject|
|Lipemia||Mild reject; Gross reject|
|Icterus||Mild reject; Gross reject|
|Specimen Type||Temperature||Time||Special Container|
The Fungitell Beta-D Glucan assay is indicated for the presumptive diagnosis of invasive fungal disease through detection of elevated levels of (1,3)- Beta-D-glucan in serum. Normal human serum contains low levels of (1,3)- Beta-D glucan, typically 10 to 40 pg/mL, presumably from commensal yeasts present in the alimentary canal and gastrointestinal tract. However, (1,3)- Beta-D-glucan is sloughed from the cell walls during the life cycle of most pathogenic fungi. Thus, monitoring serum for evidence of elevated and rising levels of (1,3)- Beta-D-glucan provides a convenient surrogate marker for invasive fungal disease.
The Fungitell Beta - D Glucan assay detects (1,3) - Beta-D-glucan from the following pathogens: Candida spp., Acremonium, Aspergillus spp., Coccidioides immitis, Fusarium spp., Histoplasma capsulatum, Trichosporon spp., Sporothrix schenckii, Saccharomyces cerevisiae, and Pneumocystis jiroveci. The Fungitell Beta-D Glucan assay does not detect certain fungal species such as the genus Cryptococcus, which produces very low levels of (1,3) - Beta-D-glucan, nor the Zygomycetes, such as Absidia, Mucor, and Rhizopus, which are not known to produce (1,3) - Beta-D-glucan. Studies indicate Blastomyces dermatitidis is usually not detected due to little (1,3) - Beta-D-glucan produced in the yeast phase.
A reference range for specimens other than serum has not been established.
The performance characteristics of the Fungitell assay in CSF have been determined by Viracor-IBT Laboratories; there are no established criteria for the interpretation of Fungitell results from CSF fluid. Research studies have evaluated the use of the Fungitell assay in CSF during a fungal meningitis outbreak (J. Clin. Microbiol. 2013, 51(4):1285-1287).
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