Test Id : CULFB
Fibroblast Culture for Biochemical or Molecular Testing, Chorionic Villi/Products of Conception/Tissue
Useful For
Suggests clinical disorders or settings where the test may be helpful
Producing cell cultures that can be used for enzymatic or molecular genetic analysis
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
This processing test is for culturing chorionic villi, products of conception, skin fibroblasts, or other biopsy specimens for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.
When this test is ordered, cryopreservation will be performed. For multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered. Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.
To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.
If viable cells are not obtained within 30 days, client will be notified.
If Mycoplasma is detected, all pending fibroblast clinical testing will be canceled, and samples will be discarded.
Method Name
A short description of the method used to perform the test
Cell Culture
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
Cell culture, fibroblasts
Culture for Genetic Testing, Chorionic Villus/CVS
Culture for Genetic Testing, Fibroblasts
Culture for Genetic Testing, Products of Conception/Stillbirth/Autopsy
Culture for Genetic Testing, Tissue
Fibroblast culture for genetic testing
Genetic Testing Culture, Chorionic Villus/CVS
Genetic Testing Culture, Fibroblasts
Genetic Testing Culture, Tissue
Genetic Testing, Culture, Products of Conception/Stillbirth/Autopsy
Saving Cells for Possible Genetic Testing
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
This processing test is for culturing chorionic villi, products of conception, skin fibroblasts, or other biopsy specimens for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.
When this test is ordered, cryopreservation will be performed. For multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered. Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.
To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.
If viable cells are not obtained within 30 days, client will be notified.
If Mycoplasma is detected, all pending fibroblast clinical testing will be canceled, and samples will be discarded.
Specimen Type
Describes the specimen type validated for testing
Tissue
Ordering Guidance
This test is most useful for chorionic villus sampling, products of conception, and skin biopsy specimens. The specimen is cryopreserved for a minimum of 6 months.
If ordering this test to obtain DNA for testing at a different laboratory location, see Necessary Information.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service
Necessary Information
1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.
2. Indicate the tests to be performed on the fibroblast culture cells.
Note: For testing performed on fibroblasts at locations other than Mayo Clinic Laboratories (MCL), a MCL Test ID and test requisition for the requested laboratory are required. Prior to specimen collection, call 800-533-1710 to confirm availability of send-out testing options. Courtesy DNA extraction on fibroblast cultures will not be performed for tests not in the MCL referral catalog.
3. Final Disposition of Fetal/Stillborn Remains is required for products of conception or stillbirth specimens.
ORDER QUESTIONS AND ANSWERS
| Question ID | Description | Answers |
|---|---|---|
| CG770 | Reason for Referral | |
| CG899 | Specimen |
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Submit only 1 of the following specimens:
Specimen Type: Autopsy
Supplies: Hank's Solution (T132)
Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline
Specimen Volume: 4-mm diameter
Collection Instructions:
1. Wash biopsy site with an antiseptic soap.
2. Thoroughly rinse area with sterile water.
3. Do not use alcohol or iodine preparations.
4. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.
Specimen Type: Chorionic villi
Supplies: CVS Media (RPMI) and Small Dish (T095)
Container/Tube: 15 mL tube containing 15 mL of transport media
Specimen Volume: 20-30 mg
Collection Instructions:
1. Collect specimen by the transabdominal or transcervical method.
2. Transfer the chorionic villi specimen to a Petri dish containing transport medium.
3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal decidua.
Specimen Type: Products of conception or stillbirth
Supplies: Hank's Solution (T132)
Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline
Specimen Volume: 1 cm(3) of placenta (including 20 mg of chorionic villi) and a 1 cm(3) biopsy specimen of muscle/fascia from the thigh
Collection Instructions: If a fetus cannot be specifically identified, collect 50 mg villus material or tissue that appears to be of fetal origin.
Additional Information: Do not send entire fetus.
Specimen Type: Cultured fibroblasts
Container/Tube: T-25 flask
Specimen Volume: 2 Full T-25 flasks
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Skin biopsy
Supplies: Fibroblast Biopsy Transport Media (T115)
Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin).
Specimen Volume: 4-mm punch
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Forms
1. Biochemical Genetics Patient Information (T602)
2. Final Disposition of Fetal/Stillborn Remains is required for products of conception or stillbirth specimens.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
See Specimen Required
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Tissue | Refrigerated (preferred) | ||
| Ambient | |||
Useful For
Suggests clinical disorders or settings where the test may be helpful
Producing cell cultures that can be used for enzymatic or molecular genetic analysis
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
This processing test is for culturing chorionic villi, products of conception, skin fibroblasts, or other biopsy specimens for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.
When this test is ordered, cryopreservation will be performed. For multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered. Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.
To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.
If viable cells are not obtained within 30 days, client will be notified.
If Mycoplasma is detected, all pending fibroblast clinical testing will be canceled, and samples will be discarded.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Cultured cells may be used to perform a wide range of laboratory tests. Prior to testing, the tissue may need to be cultured to obtain adequate numbers of cells.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Not applicable
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Interfering factors:
-Inadequate amount of specimen may not permit adequate analysis
-Exposure of the specimen to temperature extremes (freezing or greater than 30 degrees C) may kill cells and interfere with attempts to culture cells
-Improper packaging may result in broken, leaky, and contaminated specimens during transport
-Transport time should not exceed 2 days
-Contamination by maternal cells may interfere with attempts to culture cells and may cause interpretive problems
Lack of viable cells
-Bacterial contamination
-Failure to transport tissue in an appropriate media
-Excessive transport time
-Exposure of the specimen to temperature extremes (freezing or >30 degrees C)
-Improper packaging may result in broken or leaky specimen containers or contamination of specimens during transport
Clinical Reference
Recommendations for in-depth reading of a clinical nature
Arsham MS, Barch MJ, Lawce HJ, eds. The AGT Cytogenetics Laboratory Manual. 4th ed. John Wiley and Sons Inc; 2017
Method Description
Describes how the test is performed and provides a method-specific reference
Products of Conception/Autopsy/Stillbirth/Skin Biopsy:
The biopsy specimen is cut into small pieces, treated with collagenase, and placed in a tissue culture flask with Chang and MEM alpha-medium, 20 percent fetal bovine serum, and antibiotics to establish a fibroblast culture. The cultures are trypsinized into 1 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks.
Chorionic Villi Specimen:
The chorionic villi specimen is thoroughly cleaned using sterile forceps to remove the remaining maternal decidua and blood clots. The villi are then treated with trypsin and collagenase. The cells are grown in Chang and MEM-alpha medium for 5 to 10 days.(May KM, Saxe DF, Priest JH. Prenatal chromosome diagnosis. In: Arsham MS, Barch MJ, Lawce HJ, eds. The AGT Cytogenetics Laboratory Manual. 4th ed. John Wiley and Sons Inc; 2017:182-184)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
Not Applicable
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
88233
88240
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| CULFB | Fibroblast Culture for Genetic Test | 96300-9 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| 52327 | Result Summary | 50397-9 |
| 52329 | Interpretation | 69965-2 |
| 52328 | Result | 82939-0 |
| CG770 | Reason for Referral | 42349-1 |
| CG899 | Specimen | 31208-2 |
| 52331 | Source | 31208-2 |
| 52332 | Method | 85069-3 |
| 54625 | Additional Information | 48767-8 |
| 52333 | Released By | 18771-6 |