Test Id : ABTIR
Antibody Titer, Blood and Serum
Useful For
Suggests clinical disorders or settings where the test may be helpful
Monitoring antibody levels during pregnancy to help assess the risk of hemolytic disease of the newborn
This test is not useful for monitoring the efficacy of Rh-immune globulin administration.
Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.
| Test Id | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| DCTR | Direct Antiglobulin Test (Poly) | Yes | No |
| SPAGR | Special Red Cell Ag Typing | Yes | No |
| ABIDR | Antibody Identification, RBC | Yes | No |
| ABTIR | Antibody Titer, RBC | Yes | No |
| STTX25 | Antibody Elution | No, (Bill Only) | No |
| STTX31 | Antibody Adsorption | No, (Bill Only) | No |
| STTX32 | Red Cell Antigen Typing | No, (Bill Only) | No |
Additional Tests
Lists tests that are always performed, at an additional charge, with the initial tests.
| Test Id | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| STTX26 | Antibody Panel | No, (Bill Only) | Yes |
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
If the antibodies detected are too weakly reactive to titer, this test will be canceled and the antibody identification will be ordered and performed at an additional charge.
Method Name
A short description of the method used to perform the test
Hemagglutination
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
ABTIR
Antibody Titer, RBC
Allo Titer
Antibody Titer, Erythrocytes
RBC Antibody Titer
Rh (Rhesus) Titer
Anti-D Titer
Anti-E Titer
Anti-C Titer
Anti-Fya Titer
Anti-Fyb Titer
Duffy Antibody Titer
Kell Antibody Titer
Anti-K Titer
Anti-Jka Titer
Anti-Jkb Titer
Kidd Antibody Titer
Anti-e
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
If the antibodies detected are too weakly reactive to titer, this test will be canceled and the antibody identification will be ordered and performed at an additional charge.
Specimen Type
Describes the specimen type validated for testing
Varies
Shipping Instructions
Specimen must arrive within 72 hours of collection.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Blood cells, plasma, and serum are required.
Supplies: Sarstedt Aliquot Tube, 5mL (T914)
Specimen Type: Plasma/Blood
Collection Container/Tube: 6-mL Pink top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume:
3 mL Plasma
3 mL Red blood cells (RBCs)
Collection Instructions:
1. Centrifuge and aliquot plasma into plastic vial.
2. Label specimens as EDTA plasma or EDTA RBCs as appropriate.
3. Send both tubes.
Specimen Type: Serum/Blood
Collection Container/Tube: 10-mL Red top
Submission Container/Tube: Plastic vial
Specimen Volume:
5 mL Serum
5 mL RBCs
Collection Instructions:
1. Centrifuge and aliquot serum into a plastic vial.
2. Label specimens as serum or clotted RBCs as appropriate.
3. Send both tubes.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
Blood: 6 mL EDTA
Pediatric: 2 mL Serum
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | 4 days | |
| Refrigerated | 4 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Monitoring antibody levels during pregnancy to help assess the risk of hemolytic disease of the newborn
This test is not useful for monitoring the efficacy of Rh-immune globulin administration.
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
If the antibodies detected are too weakly reactive to titer, this test will be canceled and the antibody identification will be ordered and performed at an additional charge.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Some maternal IgG alloantibodies to red blood cell antigens will cross the placenta and cause hemolysis of antigen-positive fetal red blood cells. The resulting fetal anemia and hyperbilirubinemia can be harmful or possibly fatal to the newborn.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative
If positive, result will be reported as the reciprocal of the highest dilution at with macroscopic agglutination (1+) is observed.
Interpretation
Provides information to assist in interpretation of the test results
The specificity of the maternal alloantibody will be stated. The titer result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed.
If the antibody problem identified is not relevant in hemolytic disease of the newborn or if titrations are not helpful, the titer will be canceled and will be replaced by ABIDR / Antibody Identification, Blood and Serum.
A consultation service is offered, at no charge, regarding the clinical relevance of red blood cell antibodies.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Recent administration of Rh-immune globulin may cause anti-D to be identified and appear falsely as an alloantibody.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
Cohn CS, Delaney M, Johnson ST, Katz LM, Schwartz J. eds: Technical Manual. 21st ed. AABB; 2023
Method Description
Describes how the test is performed and provides a method-specific reference
The strength and specificity of the antibody to be titered is first determined. Two-fold serial dilutions of serum are tested against antigen-positive erythrocytes. The result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed at the antihuman globulin phase of testing. Parallel titration of a previous specimen of the patient's serum (frozen) provides a baseline for comparison of antibody level. In the absence of a previous specimen from the patient, parallel titration of a control antiserum is used for standardization.(Cohn CS, Delaney M, Johnson ST, Katz LM, Schwartz J. eds: Technical Manual. 21st ed. AABB; 2023)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday, Sunday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
86886
86870 (if appropriate-per panel tested)
86860 (if appropriate)
86880 x 3 (if appropriate)
86905- (if appropriate)
86978 (if appropriate)
81403 (if appropriate)-Internal only
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| ABTIR | Antibody Titer, RBC | 50962-0 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| SPECR | Antibody Specificity | 888-8 |
| ALTIR | Allo Antibody Titer | In Process |
| CTPSR | Current Titer of Previous Specimen | In Process |