Test Catalog

Test ID: ALAD    
Aminolevulinic Acid Dehydratase, Whole Blood

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

The following algorithms are available in Special Instructions:

-Porphyria (Acute) Testing Algorithm

-Porphyria (Cutaneous) Testing Algorithm

-The Heme Biosynthetic Pathway

Specimen Type Describes the specimen type validated for testing

Whole blood

Ordering Guidance

This assay is not useful in assessment of lead intoxication as it reactivates aminolevulinic acid dehydratase that has been inhibited by lead. The preferred test for lead toxicity is PBDB / Lead with Demographics, Blood.

Necessary Information

Include a list of medications the patient is currently taking.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Patient Preparation: Abstinence from alcohol is essential for at least 24 hours prior to specimen collection as ethanol suppresses aminolevulinic acid dehydratase (ALAD) activity, leading to false-positive results.


Preferred: Green top (sodium heparin)

Acceptable: Lavender top (EDTA) or green top (lithium heparin)

Specimen Volume: Full tube 4 mL

Collection Instructions: Immediately place specimen on wet ice.

Special Instructions Library of PDFs including pertinent information and forms related to the test


New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:

-Informed Consent for Genetic Testing (T576)

-Informed Consent for Genetic Testing-Spanish (T826)

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

3 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Whole bloodRefrigerated (preferred)7 days
 Ambient 4 days