Referred Tests
| Web: | mayocliniclabs.com |
|---|---|
| Email: | mcl@mayo.edu |
| Telephone: | 800-533-1710 |
| International: | +1 855-379-3115 |
| Values are valid only on day of printing. | |
Test ID: ACASM
Pernicious Anemia Cascade, Serum
Testing Algorithm 
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
If vitamin B12 is <150 ng/L, then intrinsic factor blocking antibody (IFBA) is performed. If IFBA is negative or indeterminate, then gastrin is performed.
If vitamin B12 is 150 to 400 ng/L, then methylmalonic acid (MMA) is performed. If MMA is >0.40 nmol/mL, then IFBA is performed.
See Vitamin B12 Deficiency Evaluation in Special Instructions.
Specimen Type Describes the specimen type validated for testing
Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 4 mL
Collection Instructions:
1. Fasting (8 hours)
2. Divide specimen into 3 plastic vials, 1 containing 1 mL, 1 containing 1.5 mL, and 1 containing 1.5 mL
3. Band specimens together.
Additional Information: This test should not be ordered on patients who have received vitamin B12 injection within the last 2 weeks.
Special Instructions Library of PDFs including pertinent information and forms related to the test
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.
Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Gross hemolysis | Reject |
| Gross lipemia | OK |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen | 14 days |