TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: TLBLF    
T-Lymphoblastic Leukemia/Lymphoma, FISH, Tissue

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test may be ordered in 2 distinct ways allowing different combinations of probes to be utilized based on the clinical question, including the standard (diagnostic) TLBL FISH panel and the individual TLBL FISH probes (per client request).

 

The specific TLBL FISH panel or probes requested must be noted on the request form or in the reason for referral. If no specific panel or FISH probes are indicated, the standard (diagnostic) panel will be performed.

 

The standard (diagnostic) panel includes testing for the following abnormalities, using the FISH probes listed:

-1p33 rearrangements, TAL1/STIL(SIL)

-t(5;14)(q35;q32), TLX3(HOX11L2)/BCL11B

-7q34 rearrangements, TRB(TCR beta)

-9p-, CDKN2A(p16)/D9Z1

-t(9;22)(q34;q11.2), ABL1 amplification, ABL1(ABL)/BCR

-t(10;11)(p12;q14), MLLT10(AF10)/PICALM

-11q23 rearrangements, MLL

-14q11.2 rearrangements, TRAD (TCR alpha delta)

-17p-, TP53/D17Z1

 

When an MLL rearrangement is identified, reflex testing will be performed with 1 or more dual-fusion probe sets (D-FISH) in an attempt to identify the translocation partner. Probes include:

-t(4;11)(q21;q23), AFF1(AF4)/MLL

-t(6;11)(q27;q23), MLLT4(AF6)/MLL

-t(9;11)(p22;q23), MLLT3(AF9)/MLL

-t(10;11)(p12;q23), MLLT10(AF10)/MLL

-t(11;19)(q23;p13.1), MLL/ELL

-t(11;19)(q23;p13.3), MLL/MLLT1(ENL)

 

When an TRB(TCR beta) rearrangement is identified, reflex testing will be performed with a dual-fusion probe sets

(D-FISH) in an attempt to identify the translocation partner.

-t(7;10)(q34;q24), TRB(TCR beta)/TLX1(HOX11)

 

When an TRAD(TCR alpha delta) rearrangement is identified, reflex testing will be performed with a dual-fusion probe sets

(D-FISH) in an attempt to identify the translocation partner.

-t(10;14)(q24;q11.2), TLX1(HOX11)/TRAD(TCR alpha delta)

 

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

Specimen Type Describes the specimen type validated for testing

Tissue

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:

 

Specimen Type: Tissue

Container/Tube: Formalin-fixed, paraffin-embedded tumor tissue block

 

Specimen Type: Slides

Specimen Volume: 19 Consecutive, unstained, 5 micron-thick sections placed on positively charged slides and 1 hematoxylin and eosin-stained slide

Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Formalin-fixed paraffin-embedded tissue block or for each probe set ordered, 9 unstained consecutive tissue sections cut at 5 microns and placed on positively charged microscope slides. Include 1 hematoxylin and eosin (H and E) stained slide.

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
TissueAmbient (preferred)
 Refrigerated