TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: AHUSD    
Atypical Hemolytic Uremic Syndrome (aHUS) Complement Panel, Serum and Plasma

Specimen Type Describes the specimen type validated for testing

Plasma Na Cit
Serum Red

Ordering Guidance

This test should be performed prior to treatment initiation and in the absence of therapy with complement inhibitors, such as eculizumab or ravulizumab. Complement inhibitors will affect performance of these assays.

 

For evaluating patients with possible thrombotic microangiopathies (TMA), the recommended first-tier test is ADM13 / ADAMTS13 Activity and Inhibitor Profile, Plasma. This test, AHUSD, should be a second-tier test for TMA.

 

For patients who have received eculizumab or need to monitor response to eculizumab therapy, the recommended test is ECUMP / Eculizumab Monitoring Panel, Serum. Soluble membrane attack complex (sMAC) should not be used as a standalone assay to monitor eculizumab efficiency.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Both plasma and serum are required for this test.

 

Patient Preparation:

1. Fasting preferred.

2. Samples should not be collected earlier than 48 hours following plasma exchange.

 

Specimen Type: Plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge; 1500 x g for 10 minutes at 4 degrees C, and aliquot plasma into plastic vial.

3. Freeze specimen within 30 minutes.

 

Specimen Type: Serum

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge at 4 degrees C and aliquot serum into plastic vial.

3. Freeze specimen within 30 minutes.

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Renal Diagnostics Test Request (T830)

-Coagulation Test Request (T753)

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Serum, Plasma: 1 mL each

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis OK
Gross lipemia Reject
Gross icterus OK

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Plasma Na CitFrozen14 days
Serum RedFrozen14 days