TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: LPA3P    
Lymphocyte Proliferation to Anti-CD3/Anti-CD28 and Anti-CD3/Interleukin-2 (IL-2) by Flow Cytometry, Blood

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Testing with 2 stimulants will always be performed. When adequate specimen is available, a third stimulant will be performed and billed separately. If isolated patient’s peripheral blood mononuclear cells (PBMCs) have a low WBC, selected dilutions or stimulants may not be used at the discretion of the laboratory to ensure the most reliable results.

Specimen Type Describes the specimen type validated for testing

WB Sodium Heparin

Shipping Instructions

Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the box.

 

It is recommended that specimens arrive within 24 hours of draw.

 

Samples arriving on the weekend and observed holidays may be canceled.

Necessary Information

Date and time of draw and ordering physician's name and phone number are required.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Container/Tube: Green top (sodium heparin)

Specimen Volume:

<3 months: 1 mL

3 months-5 years: 3 mL

6-18 years: 5 mL

>18 years: 20 mL

Collection Instructions: Send specimen is original tube. Do not aliquot.

 

Blood Volume Recommendations Based on Absolute Lymphocyte Count (ALC)

 

ALC

Blood Volume for Minimum aCD28 Only

Blood Volume for Minimum of aCD3, aCD28, and IL-2

Blood Volume for Full Assay

<0.5

>15 cc

>28 cc

>50 cc

0.5-1.0

15 cc

28 cc

50 cc

1.1-1.5

6.5 cc

12 cc

24 cc

1.6-2.0

4.5 cc

8.5 cc

16 cc

2.1-3.0

3.5 cc

6.5 cc

12 cc

3.1-4.0

2.5 cc

4.5 cc

8 cc

4.1-5.0

1.8 cc

3.5 cc

6 cc

>5.0

1.5 cc

2.5 cc

5 cc

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia OK

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
WB Sodium HeparinAmbient48 hoursGREEN TOP/HEP