TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: HIVDX    
HIV-1 and HIV-2 Antigen and Antibody Diagnostic Evaluation, Plasma

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test begins with HIV-1/-2 antigen and antibody screen by chemiluminescence immunoassay. If the screen result is reactive, then HIV-1/-2 antibody differentiation test by immunochromatographic method is performed at an additional charge.

If HIV-1/-2 antibody differentiation is negative for both HIV-1 antibody and HIV-2 antibody, or indeterminate/negative for HIV-1/HIV-2 antibody, or indeterminate/indeterminate for HIV-1/ HIV-2 antibody, then the HIV-1 RNA detection and quantification is performed at an additional charge.

 

The following algorithms are available in Special Instructions:

-HIV Testing Algorithm (Fourth-Generation Screening Assay), Including Follow-up of Reactive Rapid Serologic Test Results

-Meningitis/Encephalitis Panel Algorithm

Specimen Type Describes the specimen type validated for testing

Plasma

Advisory Information

If specimen is from either autopsy or cadaver blood sources, the proper FDA-licensed assay is HV1CD / HIV-1 and HIV-2 Antibodies for Cadaveric or Hemolyzed Specimens, Serum.

 

Screening, supplemental or confirmatory serologic tests for HIV-1 or HIV-2 antibodies cannot distinguish between active neonatal HIV infection and passive transfer of maternal HIV antibodies in infants during the postnatal period (up to 2 years old). Diagnosis of HIV infection in newborns and infants up to 2 years old should be made by virologic tests, such as detection of HIV-1 DNA and RNA (HIVP / HIV-1 DNA and RNA Qualitative Detection by PCR, Plasma) or HIV-1 RNA (HIVQN / HIV-1 RNA Detection and Quantification, Plasma).

 

New York State clients: This test should not be requested for maternal/newborn HIV screening on specimens originating in New York State, due to state regulatory requirements for expedited result reporting.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 4 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot plasma into a plastic vial.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1.2 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
PlasmaRefrigerated (preferred)6 days
 Frozen 30 days