TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: TCP    
T-Cell Subsets, Naive, Memory, and Activated, Blood

Method Description Describes how the test is performed and provides a method-specific reference

This flow cytometric assay quantitates the following CD4 and CD8 T-cell subsets: naive (global and CD62L+), memory (global, central, and effector memory), and activated (CD4+25+ and major histocompatibility [MHC] class II-positive) T cells. EDTA anticoagulated blood is incubated with antibodies to various T-cell markers (ie, CD3, CD4, CD8, CD45RO, CD45RA, HLADR, CD27, CD62L, CD25, and CD28). After RBC lysis, the sample is washed to remove any unbound antibodies.Each T-cell subset is expressed as a percentage of total CD4+ or CD8 T cells. Only the CD3 T cells are expressed as a percentage of total lymphocytes. The absolute counts for the T-cell subsets are derived from flow cytometry analysis of whole blood using monoclonal antibodies to identify CD45, CD3, CD4, and CD8. The T-cell subsets panel is linked to the TCD4 test (TCD4 / CD4 Count for Immune Monitoring, Blood) within the experiment and, therefore, the CD3, CD4, and CD8 T-cell reference ranges are provided within the TCD4 assay. The results for the other T-cell subsets are interpreted using a reference range derived from data of normal healthy adult and pediatric donors. Isotype controls are used in each assay to measure background fluorescence of the samples. A normal, healthy control is also included in each experiment to ensure the optimal performance of the assay.(Unpublished Mayo information)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Specimens are required to be received in the lab weekdays and by 4 p.m. on Friday. No weekend processing.

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

3 to 4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

4 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester