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Blood samples are subjected to protein precipitation. The resulting supernatant is analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).(Charlson JC, Moyer TP: Therapeutic drug monitoring. In Tietz Textbook of Clinical Chemistry. Fourth edition. Edited by CA Burtis, ER Ashwood, DE Bruns. New York, WB Saunders Company, 2004)
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