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Samples are spiked with deuterated internal standard and are hydrolyzed overnight with acid. Samples are then neutralized and extracted by solid phase extraction (SPE). The extracts are dried, reconstituted, and analyzed by liquid chromatography - tandem mass spectrometry.(Taylor RL, Singh RJ: Validation of liquid chromatography-tandem mass spectrometry method for analysis of urinary conjugated metanephrine and normetanephrine for screening of pheochromocytoma. Clin Chem. 2002 Mar;48[3]:533-539; Wurth R, Tirosh A, Kamilaris CDC, et al. Volumetric modeling of adrenal gland size in primary bilateral macronodular adrenocortical hyperplasia. J Endocr Soc. 2020 Oct 29;5(1):bvaa162)
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