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Pyruvate, in the presence of excess NADH, H+, and lactic dehydrogenase, is reduced to lactate. The reaction is stoichiometric; the decrease in absorbance at 340 nm is directly proportional to the concentration of pyruvate.(Huckabee WE: Relationships of pyruvate and lactate during anaerobic metabolism. I. Effects of infusion of pyruvate or glucose and of hyperventilation. J Clin Invest 1958;37:244-254; Benoist J, Alberti C, Leclercq S, et al: Cerebrospinal fluid lactate and pyruvate concentrations and their ratio in children: age-related reference intervals. Clin Chem 2003;49[3]:487-494; Cowan T, Pasquali M: Laboratory Investigations of Inborn Errors of Metabolism. In Pediatric Endocrinology and Inborn Errors of Metabolism. Second Edition. Edited by K Sarafoglou, GF Hoffman, KS Roth. 2017, pp 1139-1158)
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