TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: BILHA    
Schistosoma species Antibody, IgG, Serum

Method Description Describes how the test is performed and provides a method-specific reference

The qualitative immunoenzymatic determination of IgG-class antibodies against Schistosoma mansoni is based on the ELISA technique.

 

Microtiter strip wells are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP)-labelled Protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine (TMB) substrate, which gives a blue reaction product.

 

The intensity of this product is proportional to the amount of Schistosoma-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.(Package insert: NovaLisa Schistosoma mansoni, NovaTec Immundiagnostica GmbH, Dietzenbach, Germany; 05/2017)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Tuesday, Thursday; 9 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

5 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester