Test Catalog

Test ID: SPEP    
Electrophoresis, Protein, Serum

Useful For Suggests clinical disorders or settings where the test may be helpful

Screening patients with suspected monoclonal gammopathies


Diagnosis of monoclonal gammopathies, when used in conjunction with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and free light chain analysis

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
TPETotal ProteinYes, (Order TP)Yes
SPEProtein ElectrophoresisNoYes

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
IFXEDImmunofixation Delta and Epsilon, SYesNo
MPTSM-protein Isotype MALDI-TOF MS, SYes, (Order MALD)No

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test includes total protein and serum protein electrophoresis.


If a discrete electrophoresis band is identified, the laboratory will evaluate the serum protein electrophoresis and, if necessary, perform M-protein isotype at an additional charge.


If a light chain is identified without a corresponding heavy chain during initial testing, immunofixation with IgD and IgE antisera will be performed at an additional charge.


The following algorithms are available in Special Instructions:

-Laboratory Approach to the Diagnosis of Amyloidosis

-Laboratory Screening Tests for Suspected Multiple Myeloma

Special Instructions Library of PDFs including pertinent information and forms related to the test

Method Name A short description of the method used to perform the test

TPE: Colorimetric, Biuret

SPE: Agarose Gel Electrophoresis

NY State Available Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name Lists a shorter or abbreviated version of the Published Name for a test

Electrophoresis, Protein, S

Aliases Lists additional common names for a test, as an aid in searching

Protein Electrophoresis
Immunology Profile
Serum immunoglobulins
Protein Electrophoresis +Total Protein
SPE (Serum Protein Electrophoresis)
SPEP (Serum Protein Electrophoresis)