Test Catalog

Test Id : IDENT

Organism Referred for Identification, Aerobic Bacteria

Useful For
Suggests clinical disorders or settings where the test may be helpful

Identification of pure isolates of aerobic bacteria

 

Differentiation of members of the Staphylococcus aureus complex (S aureus, S argenteus, S schweitzeri)

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
COMM Identification Commercial Kit No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SALS Serologic Agglut Method 1 Ident No, (Bill Only) No
EC Serologic Agglut Method 2 Ident No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
STAP Identification Staphylococcus No, (Bill Only) No
STRP Identification Streptococcus No, (Bill Only) No
SIDC Ident Serologic Agglut Method 4 No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged. All aerobically growing bacteria submitted will be identified and billed as appropriate.

Note: Mayo Clinic Laboratories recommends referring suspect select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) to your state health department or the CDC for identification confirmation or exclusion.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Dependent on organism submitted, 1 or more of the following methods will be used: conventional biochemical testing, commercial identification strips or panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry, and 16S RNA gene sequencing.

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Organism Refer for ID, Aerobic Bact

Aliases
Lists additional common names for a test, as an aid in searching

50012-IDENT

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged. All aerobically growing bacteria submitted will be identified and billed as appropriate.

Note: Mayo Clinic Laboratories recommends referring suspect select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) to your state health department or the CDC for identification confirmation or exclusion.

Specimen Type
Describes the specimen type validated for testing

Varies

Ordering Guidance

If susceptibility testing is needed; also order ZMMLS Antimicrobial Susceptibility, Aerobic Bacteria, MIC. If susceptibilities are not appropriate and will not be performed, ZMMLS will be canceled at report time.

Shipping Instructions

1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.

Necessary Information

1. Specimen source is required.

2. Isolate description is required including: Gram stain reaction, morphology, and tests performed.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
Q00M0032 Specimen Source (Required) and Isolate Description-Gram reaction, morphology, tests performed (Required)

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Supplies: Infectious Container, Large (T146)

Specimen Type: Pure culture of organism from source cultured

Container/Tube: Agar slant or other appropriate media

Specimen Volume: Entire specimen

Collection Instructions:

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

NA

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Other Agar plate

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
Refrigerated

Useful For
Suggests clinical disorders or settings where the test may be helpful

Identification of pure isolates of aerobic bacteria

 

Differentiation of members of the Staphylococcus aureus complex (S aureus, S argenteus, S schweitzeri)

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged. All aerobically growing bacteria submitted will be identified and billed as appropriate.

Note: Mayo Clinic Laboratories recommends referring suspect select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) to your state health department or the CDC for identification confirmation or exclusion.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Organisms are referred to confirm identification or when the identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.

 

Techniques employed may include conventional biochemical analysis, commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry or sequencing nucleic acid of the 16S ribosomal RNA (rRNA) gene.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Identification of organism

Interpretation
Provides information to assist in interpretation of the test results

Genus and species are reported on aerobic bacterial isolates, whenever possible.

 

Bacillus species will be reported out as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless species identification is specifically requested on the request form.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Isolates suspected of being select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) should be submitted to client’s state health department or the CDC for identification confirmation or exclusion.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller.. Washington, DC, ASM Press, 2019

2. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Seventh edition. Edited by GW Procop, DL Church, GS Hall, et al. Philadelphia, Lippincott Williams and Wilkins. 2017

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

Pure isolates of aerobic organisms received on slants are inoculated onto culture plates based on Gram-stain morphology, the source of the isolate, clinical history, and previous results submitted by the referring client. In general, routine media utilized includes chocolate blood agar, sheep blood agar, and eosin methylene blue agar. After incubation at 35 degrees C in 5% CO(2), the organism is identified using one or a combination of the following techniques: commercial identification strips or panels, MALDI-TOF mass spectrometry, conventional biochemical tests, and nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene.(RS Weyant, CW Moss, RE Weaver, et al: Identification of unusual pathogenic gram negative aerobic and facultatively anaerobic bacteria. CDC, Atlanta, GA, 1996; Bergey's Manual of Systematic Bacteriology, Vol 1. Edited by NR Krieg. Baltimore, MD, Williams and Wilkins, 1984; Kolbert CP, Persing DH: Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 1999;2:299-305)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

5 to 10 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

30 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87077-Organism Referred for Identification, Aerobic Bacteria

87077-Identification Commercial Kit (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)

87147-Serologic Agglut Method 2 Ident (if appropriate)

87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)

87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)

87077-Identification Staphylococcus (if appropriate)

87077-Identification Streptococcus (if appropriate)

87798-Identification by PCR (if appropriate)

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
IDENT Organism Refer for ID, Aerobic Bact 32367-5
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
IDENT Organism Refer for ID, Aerobic Bact 32367-5

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports