Test Catalog

Test ID: HBELC    
Hemoglobin Electrophoresis Cascade, Blood

Useful For Suggests clinical disorders or settings where the test may be helpful

Diagnosis and comprehensive classification of thalassemias and hemoglobin variants

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
A2FHemoglobin A2 and FNoYes
HBELHemoglobin Electrophoresis, BNoYes

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
SDEXHemoglobin S, Scrn, BYesNo
IEFIEF ConfirmsNoNo
MASSHb Variant by Mass Spec, BNoNo
UNHBUnstable Hemoglobin, BNoNo
ATHALAlpha-Globin Gene AnalysisYesNo
WASQRAlpha Globin Gene Sequencing, BYes, (Order WASEQ)No
WBSQRBeta Globin Gene Sequencing, BYes, (Order WBSEQ)No
WBDDRBeta Globin Cluster Locus Del/Dup,BYes, (Order WBDD)No
WGSQRGamma Globin Full Gene SequencingYes, (Order WGSEQ)No
HBELAHGB Electrophoresis Summary InterpNoNo
HPFHHemoglobin F, Red Cell Distrib, BNoNo

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Hemoglobin electrophoresis cascade will always include hemoglobin A(2) and F and hemoglobin electrophoresis utilizing cation exchange HPLC and capillary electrophoresis methods.

 

Hemoglobin electrophoresis reflex testing, performed at additional charge, may include any or all of the following to identify rare hemoglobin variants present: sickle solubility (hemoglobin S screen), hemoglobin heat and isopropanol stability studies (unstable hemoglobin), isoelectric focusing, intact globin chain mass spectrometry (hemoglobin variant by mass spectrometry), Hb F distribution by flow cytometry (hemoglobin F red cell distribution), DNA (Sanger) testing for beta chain variants and the most common beta thalassemias (beta-globin gene sequencing), multiplex ligation-dependent probe amplification (MLPA) testing for beta cluster locus large deletions and duplications, including large deletional hereditary persistence of fetal hemoglobin (HPFH), delta-beta (DBT), delta thalassemias, gamma-delta-beta (GDBT), and epsilon-gamma-delta-beta (EGDBT) thalassemias (beta globin cluster locus del/dup), large deletional alpha thalassemias and alpha gene duplications (alpha-globin gene analysis), alpha chain variants and non-deletional alpha thalassemias (alpha-globin gene sequencing), and gamma chain variants and non-deletional HPFH (gamma globin full gene sequencing).

 

If a Thalassemia/Hemoglobinopathy Patient Information sheet (T358) is received with the sample, the reported clinical features or clinical impression will be considered in the interpretation and focus of the evaluation. Our laboratory has extensive experience in hemoglobin variant identification and many cases can be confidently classified without molecular testing. However, molecular confirmation is always available. If no molecular testing or, conversely, specific molecular tests are desired, utilize the appropriate check boxes on the information sheet. If the information sheet or other communication is not received, the reviewing hematopathologist will select appropriate tests to sufficiently explain the clinical impression or reported CBC results, which may or may not include molecular testing.

 

Hemoglobin (HGB) Electrophoresis Summary Interpretation, an additional consultative interpretation that summarizes all testing, will be provided after test completion to incorporate subsequent results into an overall evaluation if 1 or more of the following molecular tests are reflexed on the HBELC / Hemoglobin Electrophoresis Cascade, Blood:

-ATHAL / Alpha-Globin Gene Analysis

-WASQR / Alpha-Globin Gene Sequencing, Blood

-WBSQR / Beta-Globin Gene Sequencing, Blood

-WBDDR / Beta-Globin Cluster Locus Deletion/Duplication, Blood

-WGSQR / Gamma-Globin Full Gene Sequencing

 

See Benign Hematology Evaluation Comparison in Special Instructions.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Method Name A short description of the method used to perform the test

A2F: Cation Exchange/High-Performance Liquid Chromatography (HPLC)

HBEL: Capillary Electrophoresis

IEF: Isoelectric Focusing

MASS: Mass Spectrometry (MS)

HPFH: Flow Cytometry

UNHB: Isopropanol and Heat Stability

HBELA: Consultative Interpretation

NY State Available Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name Lists a shorter or abbreviated version of the Published Name for a test

HGB Electrophoresis Cascade

Aliases Lists additional common names for a test, as an aid in searching

A2 Hemoglobin
Alpha Globin Variant
Barts Hemoglobin
Beta Globin Variant
Hemoglobin A2
Hemoglobin Cascade
Hemoglobin Electrophoresis Cascade Level 1
Hemoglobin Molecular studies
Hemoglobin Variant
HGB (Hemoglobin) Electrophoresis
Isoelectric Focusing
Sickling Test
Alpha Thalassemia
Mass Spectrometry
H Disease
Microcytosis
Sickle cell
Thalassemia
Beta Thalassemia