Test Id : LEBV
Epstein-Barr Virus (EBV), Molecular Detection, PCR, Varies
This test is only orderable for clients who send specimens directly to MCL in Jacksonville, FL. All other clients, see Rochester Test EBVPV.
Useful For
Suggests clinical disorders or settings where the test may be helpful
Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens
Diagnosis of disease due to EBV
This test should not be used to screen asymptomatic patients.
Method Name
A short description of the method used to perform the test
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
EBV (Epstein-Barr Virus)
EBV Detection by PCR (Polymerase Chain Reaction)
EBV Detection by PCR (Polymerase Chain Reaction), Spinal Fluid
EBV Detection by Real-Time PCR
Epstein-Barr Virus Detection by PCR (Polymerase Chain Reaction
Epstein-Barr Virus Detection by PCR (Polymerase Chain Reaction), CSF
Epstein-Barr Virus Detection by Polymerase Chain Reaction (PCR)
Epstein-Barr Virus Detection by Polymerase Chain Reaction (PCR), CSF
Epstein-Barr Virus Detection by Real-Time PCR
Infectious Mononucleosis
LightCycler EBV
PCR (Polymerase Chain Reaction)
PCR, Epstein-Barr
This test is only orderable for clients who send specimens directly to MCL in Jacksonville, FL. All other clients, see Rochester Test EBVPV.
Specimen Type
Describes the specimen type validated for testing
Varies
Necessary Information
Specimen source is required.
ORDER QUESTIONS AND ANSWERS
| Question ID | Description | Answers |
|---|---|---|
| SRC67 | Specimen Source |
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube:
Preferred: Sterile, screw cap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Respiratory fluid
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Supplies: Sarstedt Aliquot Tube, 5 mL
Container/Tube:
Preferred: Sterile, screw cap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 1.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Swab
Sources: Eye and upper respiratory (nasal, throat)
Supplies:
-Culturette (BBL Culture Swab) (T092)
-M4-RT (T605)
Container/Tube: Multimicrobe media (M4-RT) and Eswabs
Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4 or M5)
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA)
Specimen Volume: 0.5 mL
Additional Information: Clotted specimens will be rejected.
Specimen Type: Tissue
Sources: Brain, colon, kidney, liver, lung, cornea, etc.
Supplies:
Container/Tube:
Preferred: Multimicrobe medium (M4-RT)
Acceptable: Sterile container containing 1 to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4 or M5)
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
Fluids: 0.3 mL; Respiratory Specimens: 1 mL; Tissue: 2 x 2-mm biopsy; Swab: See Specimen Required
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Calcium alginate-tipped swab Wood swab Transport swab containing gel Formalin-fixed and paraffin-embedded tissues Heat inactivated specimens | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
This test is only orderable for clients who send specimens directly to MCL in Jacksonville, FL. All other clients, see Rochester Test EBVPV.
Useful For
Suggests clinical disorders or settings where the test may be helpful
Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens
Diagnosis of disease due to EBV
This test should not be used to screen asymptomatic patients.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis, Burkitt lymphoma, and in Southern China, nasopharyngeal carcinoma. EBV-associated central nervous system (CNS) disease is most frequently associated with primary CNS lymphoma in patients with AIDS. In addition, CNS infection associated with the detection of EBV DNA can be seen in immunocompetent patients.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative
Reference values apply to all ages.
Interpretation
Provides information to assist in interpretation of the test results
Detection of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) supports the clinical diagnosis of central nervous system (CNS) disease due to the virus.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
A negative result does not eliminate the possibility of Epstein-Barr virus (EBV) infection of the central nervous system.
This assay may detect viremia or viral shedding in asymptomatic individuals. However, this assay is only to be used for patients with a clinical history and symptoms consistent with EBV infection and must be interpreted in the context of the clinical picture.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Allen UD, Preiksaitis JK. AST Infectious Diseases Community of Practice. Post-transplant lymphoproliferative disorders, Epstein-Barr virus infection, and disease in solid organ transplantation: Guidelines from the American Society of Transplantation Infectious Diseases Community of Practice. Clin Transplant. 2019;33(9):e13652. doi:10.1111/ctr.13652
2. Nowalk A, Green M. Epstein-Barr Virus. Microbiol Spectr. 2016;4(3). doi:10.1128/microbiolspec.DMIH2-0011-2015
3. AbuSalah MAH, Gan SH, Al-Hatamleh MAI, Irekeola AA, Shueb RH, Yean Yean C. Recent Advances in Diagnostic Approaches for Epstein-Barr Virus. Pathogens. 2020;9(3):226. doi:10.3390/pathogens9030226
This test is only orderable for clients who send specimens directly to MCL in Jacksonville, FL. All other clients, see Rochester Test EBVPV.
Method Description
Describes how the test is performed and provides a method-specific reference
Viral nucleic acid is extracted by the MagNA Pure automated instrument (Roche Applied Science) from clinical specimens. Primers are directed to the target gene (latent membrane protein). The LightCycler instrument amplifies and monitors by fluorescence the development of target nucleic acid sequences after the annealing step during polymerase chain reaction (PCR) cycling. This is an automated PCR system that can rapidly detect (30-40 minutes) amplicon development through stringent air-controlled temperature cycling . The detection of amplified products is based on the fluorescence resonance energy transfer (FRET) principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3' end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red 640, at the 5' end. The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product. Melting curve analysis is performed following PCR amplification. Starting at 45 degrees C, the temperature in the thermal chamber is slowly raised to 80 degrees C and the fluorescence is measured at frequent intervals. Analysis of the PCR amplification and probe melting curves is accomplished through the use of LightCycler software.(Unpublished Mayo method)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Saturday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
This test is only orderable for clients who send specimens directly to MCL in Jacksonville, FL. All other clients, see Rochester Test EBVPV.
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
87798
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| LEBV | Epstein-Barr Virus PCR | 23858-4 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| SRC67 | Specimen Source | 31208-2 |
| 81239 | Epstein-Barr Virus PCR | 23858-4 |