Test Id : FHTGF
Human Transforming Growth Factor beta 1 (TGF-b1)
Method Name
A short description of the method used to perform the test
ELISA
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
TFG-b1
Specimen Type
Describes the specimen type validated for testing
Plasma EDTA
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Platelet-free Plasma
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 mL
Collection Instructions:
1. Draw blood in a lavender top (EDTA) tube.
2. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Centrifuge plasma again at 3000 x g for 10 minutes for complete platelet removal.
3. Aliquot plasma into a plastic vial and freeze at -20 degrees C or below.
4. Send 1 mL platelet-free plasma frozen in plastic vial.
Additional Information:
For fixed speed centrifuges such as 645e: Within 30 minutes of collection, centrifuge 3 times for 10 minutes at 1600 x g while decanting the plasma each time before the next centrifugation. Aliquot plasma into a plastic vial and freeze at -20 degrees C or below.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
0.25 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Specimens with particulate matter or microbial contamination Specimens outside of listed stability Samples submitted without two unique identifiers and date of collection | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 14 days |
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Transforming growth factor (TGF) play a crucial role in tissue regeneration, cell differentiation, embryonic development, and regulation of the immune system. Transforming growth factor beta is found in hematopoietic (blood-forming) tissue and initiates a signaling pathway that suppresses the early development of cancer cells. It enhances the deposition of extracellular matrix and may play potential role in wound healing and cirrhosis formation. Many cells synthesize TGF-b and almost all of them have specific receptors for this peptide.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
0-22,062 pg/mL
Method Description
Describes how the test is performed and provides a method-specific reference
This quantitative assay employs sandwich ELISA method. Microwells are pre-coated with antibodies against TGF-b1. The diluted patient samples (after activation) are added into the wells and any TGF-b1 present remains bound to the plate. After washing the wells, peroxidase labeled anti-TGF-b1 antibodies are added. Bound conjugate is visualized with TMB substrate and intensity of color is proportional to the concentration of TGF-b1 in the sample. Stop dilution is added to each well to stop the reaction.
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Varies, 2 days per week
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
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Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
The performance characteristics of the listed assay was validated by BioAgilytix Diagnostics. The US FDA has not approved or cleared this test. The results of this assay can be used for clinical diagnosis without FDA approval. BioAgilytix Diagnostics is a CLIA certified, CAP accredited laboratory for performing high complexity assays such as this one.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
83520
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| FHTGF | Human Transforming Growth Factor b1 | Not Provided |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| Z6269 | TGF-b1 | Not Provided |