Test Id : ANPAT
Anatomic Pathology Consultation, Wet Tissue
Useful For
Suggests clinical disorders or settings where the test may be helpful
Obtaining a rapid, expert opinion on unprocessed specimens (lung tissue for immunofluorescence, cardiac biopsies, enucleated eye specimens, iris, conjunctiva, cornea, and other small eye biopsies) referred by a pathologist
This test is not useful for suspected hematologic disorders.
Highlights
Our consultative practice strives to provide the highest quality diagnostic consultative service, balancing optimal patient care with a cost-conscious approach that supports the rapid turnaround time for diagnostic results.
Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.
| Test Id | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| LEV1P | Level 1 Gross only | No, (Bill Only) | No |
| LEV2P | Level 2 Gross and microscopic | No, (Bill Only) | No |
| LEV3P | Level 3 Gross and microscopic | No, (Bill Only) | No |
| LEV4P | Level 4 Gross and microscopic | No, (Bill Only) | No |
| LEV5P | Level 5 Gross and microscopic | No, (Bill Only) | No |
| LEV6P | Level 6 Gross and microscopic | No, (Bill Only) | No |
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Laboratory approval is required prior to ordering this test. Contact Mayo Clinic Laboratories at 800-533-1710.
An interpretation will be provided by Mayo Clinic staff pathologists within a formal pathology report sent to the referring pathologist.
Appropriate stains and other ancillary testing may be performed at an additional charge.
Note: Tests requested by the referring physician (immunostains, molecular studies, etc.) may not be performed if they are deemed to be unnecessary by the reviewing Mayo Clinic pathologist. For all pathology consultations, ancillary testing is ordered at the discretion of the Mayo Clinic pathologist.
Note: Calls are not routinely made; however, depending on the nature of the case, a call may be placed to the ordering provider or pathologist. To contact a Mayo Clinic Pathologist, contact Mayo Clinic Laboratories at 800-533-1710.
For more information see Pathology Consultation Ordering Algorithm.
Method Name
A short description of the method used to perform the test
Medical Interpretation
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
ANPAT
Biopsy
Pathology Consultation
Wet Tissue
Cardiovascular autopsy
CV autopsy
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Laboratory approval is required prior to ordering this test. Contact Mayo Clinic Laboratories at 800-533-1710.
An interpretation will be provided by Mayo Clinic staff pathologists within a formal pathology report sent to the referring pathologist.
Appropriate stains and other ancillary testing may be performed at an additional charge.
Note: Tests requested by the referring physician (immunostains, molecular studies, etc.) may not be performed if they are deemed to be unnecessary by the reviewing Mayo Clinic pathologist. For all pathology consultations, ancillary testing is ordered at the discretion of the Mayo Clinic pathologist.
Note: Calls are not routinely made; however, depending on the nature of the case, a call may be placed to the ordering provider or pathologist. To contact a Mayo Clinic Pathologist, contact Mayo Clinic Laboratories at 800-533-1710.
For more information see Pathology Consultation Ordering Algorithm.
Specimen Type
Describes the specimen type validated for testing
Varies
Ordering Guidance
For a pathology consultation on paraffin embedded tissue and slides only (ie, no wet tissue being sent to be processed), order PATHC / Pathology Consultation.
Shipping Instructions
Vitreous fluid/aqueous humor specimens: ship overnight; Attention: Ophthalmic Pathology Working Group. Call 800-533-1710 to request an Expedite on this order since vitreous fluid will have no fixative and needs to reach the laboratory as quickly as possible.
Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.
Necessary Information
All requisition and supporting information must be submitted in English.
Each of the following items is required:
1. All requisitions must be labeled with:
-Patient name, date of birth and medical record number
-Name and phone number of the referring pathologist or ordering provider
-Anatomic site and collection date
2. A preliminary or final pathology report and brief patient history are essential to achieve a consultation fully relevant to the ordering provider's needs.
3. Additional information by case type
Cardiac explant: Include the last pretransplantation echocardiogram with report and images as well as all other relevant clinical documents (eg, family history of cardiac events, cardiac laboratory testing results)
Iris, conjunctiva, cornea, and other small biopsies from eye: Clinical/surgical notes should be included if available.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Submit 1 of the following specimens:
Specimen Type: Lung biopsy
Supplies:
-Pathology Packaging Kit (T554)
-Formalin, 10% Buffered Neutral for Tissue (T118)
-Michel's Transport Media for Immunofluorescent Testing on Tissue (T321)
Specimen Volume: Biopsy specimens as delineated below
Collection Instructions:
For testing to be performed, both of the following must be submitted:
a. For light microscopy:
Preferred: A portion of lung tissue in 10% neutral buffered formalin. Specimens submitted in formalin needs to be fully submerged with the optimal approximate specimen volume of 10:1 or higher.
Acceptable: A representative hematoxylin and eosin (H and E)-stained slide
b. For immunofluorescence, a portion of the unfixed lung tissue in Michel's transport media
Specimen Type: Cardiac biopsy
Supplies:
-Pathology Packaging Kit (T554)
-Formalin, 10% Buffered Neutral for Tissue (T118)
-Gluta (Trumps) (T130)
Specimen Volume: Biopsy specimens as delineated below
Collection Instructions:
1. Collect 4 to 6 biopsy specimens and submit all in 10% neutral buffered formalin. Specimens submitted in formalin needs to be fully submerged with the optimal approximate specimen volume of 10:1 or higher. If formalin biopsy specimens are not available, light microscopy slides would also be acceptable.
2. If electron microscopy (EM) is also requested, submit at least 1 biopsy into 2.5% to 3% buffered glutaraldehyde preservative (Trumps) along with the formalin biopsy specimens.
Additional Information: If the question is drug toxicity, storage disease, or if the patient is younger than 25 years, send at least 1 biopsy specimen in 2.5% to 3% buffered glutaraldehyde (Trumps) in case EM is needed for diagnosis.
Specimen Type: Cardiac explant
Supplies: Pathology Packaging Kit (T554)
Specimen Volume: Entire explant specimen
Collection Instructions:
1. Fix entire explant specimen in formalin for a minimum of 24 hours.
2. Following fixation, lightly wrap specimen in formalin-soaked paper towel, place in a biohazard bag with a small amount of formalin, seal, place in another biohazard bag, and seal.
Specimen Type: Enucleated eye
Supplies:
-Pathology Packaging Kit (T554)
-Formalin - 10% Histo Prep, 45 mL (T117)
Specimen Volume: Entire eye specimen
Collection Instructions:
1. Immediately after removal, place specimen in approximately 300 mL of 10% neutral buffered formalin and fix for 48 hours.
2. Enucleated eye should not be opened or punctured.
3. After the 48-hour fixation, the specimen should be transferred from the larger, 300 mL container to a smaller container with fixative (Formalin - 10% Histo Prep, 45 mL) for shipping.
Specimen Type: Iris, conjunctiva, cornea, and other small biopsies from eye
Supplies:
-Pathology Packaging Kit (T554)
-Formalin - 10% Histo Prep, 45 mL (T117)
Specimen Volume: Entire biopsy specimen
Collection Instructions:
1. Place iris, conjunctiva, cornea, and other small biopsies on a paper mount (a piece of filter paper or other porous paper). This helps to keep the specimen from curling when it is fixed.
2. Immediately, gently place paper-mounted specimen into 10% formalin (Formalin - 10% Histo Prep, 45 mL).
3. In cases where margins are important, placing sutures at the margins of resection with accompanying explanatory draft will help orientation of the specimen in the lab.
Specimen Type: Vitreous fluid/Aqueous humor
Supplies:
-Pathology Packaging Kit (T554)
-Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plastic screw-top vial
Specimen Volume: Entire specimen
Collection Instructions:
1. Place the liquid (vitreous or aqueous) concentrate (preferred) or washing (diluted, acceptable) into a small tube with a screw-top cap.
2. Refrigerate the specimen immediately (do not freeze).
Specimen Stability Information: Refrigerated
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
See Specimen Required
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
| Frozen | |||
Useful For
Suggests clinical disorders or settings where the test may be helpful
Obtaining a rapid, expert opinion on unprocessed specimens (lung tissue for immunofluorescence, cardiac biopsies, enucleated eye specimens, iris, conjunctiva, cornea, and other small eye biopsies) referred by a pathologist
This test is not useful for suspected hematologic disorders.
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Laboratory approval is required prior to ordering this test. Contact Mayo Clinic Laboratories at 800-533-1710.
An interpretation will be provided by Mayo Clinic staff pathologists within a formal pathology report sent to the referring pathologist.
Appropriate stains and other ancillary testing may be performed at an additional charge.
Note: Tests requested by the referring physician (immunostains, molecular studies, etc.) may not be performed if they are deemed to be unnecessary by the reviewing Mayo Clinic pathologist. For all pathology consultations, ancillary testing is ordered at the discretion of the Mayo Clinic pathologist.
Note: Calls are not routinely made; however, depending on the nature of the case, a call may be placed to the ordering provider or pathologist. To contact a Mayo Clinic Pathologist, contact Mayo Clinic Laboratories at 800-533-1710.
For more information see Pathology Consultation Ordering Algorithm.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Mayo Clinic in Rochester, Minnesota is staffed by pathologists whose expertise and special interests cover the entirety of Pathology, from surgical pathology with all its respective subspecialty areas, to Hematopathology, Renal Pathology, and Dermatopathology.
Consultation services are provided for difficult diagnostic problems. Consultation cases may be sent by a referring pathologist and directed to one of the pathologists who is an expert in the given area or directed more broadly to the subspecialty group. Cases are frequently shared and sometimes transferred between the pathologists, as deemed appropriate for the type of case or diagnostic problem encountered. Emphasis is placed on prompt and accurate results. Materials received are reviewed in conjunction with the clinical history provided, laboratory findings, radiographic findings (if applicable), and sending pathologist's report or letter. If additional special stains or studies are needed, the results are included in the final interpretive report. In some cases, electron microscopy and other special procedures are utilized as required.
A variety of ancillary studies are available (eg, cytochemistry, immunohistochemistry, immunofluorescence, electron microscopy, mass spectrometry, cytogenetics, and molecular genetics) to aid in establishing a diagnosis. These ancillary studies are often expensive and labor intensive and are most efficiently utilized and interpreted in the context of the morphologic features. The goal is to provide the highest possible level of diagnostic consultative service, while trying to balance optimal patient care with a cost-conscious approach to solving difficult diagnostic problems.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
An interpretive report will be provided.
Interpretation
Provides information to assist in interpretation of the test results
Results of the consultation are reported in a formal pathology report, which includes a description of ancillary test results (if applicable) and an interpretive comment. When the case is completed, results may be communicated by a phone call.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Clinical Reference
Recommendations for in-depth reading of a clinical nature
Renshaw AA, Gould EW. Measuring the value of review of pathology material by a second pathologist. Am J Clin Pathol. 2006;125(5):737-739. doi:10.1309/6A0R-AX9K-CR8V-WCG4
Method Description
Describes how the test is performed and provides a method-specific reference
All requests will be processed as a consultation case first. Special studies will be performed only if deemed to be diagnostically indicated. If the need for enzyme cytochemical stains, immunophenotyping, and/or immunohistochemical stains is anticipated, appropriate specimen requirements should be followed as indicated.
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
Not Applicable
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
88300 (if appropriate)
88302 (if appropriate)
88304 (if appropriate)
88305 (if appropriate)
88307 (if appropriate)
88309 (if appropriate)
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| ANPAT | Wet Tissue Pathology Consult | In Process |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| 71043 | Interpretation | 60570-9 |
| 71044 | Participated in the Interpretation | No LOINC Needed |
| 71045 | Report Electronically signed by | 19139-5 |
| 71046 | Addendum | 35265-8 |
| 71047 | Gross Description | 22634-0 |
| 71048 | Material Received | 22633-2 |
| 71584 | Disclaimer | 62364-5 |
| 71790 | Case Number | 80398-1 |