Test Id : IMPLF
Immature Platelet Fraction, Blood
Useful For
Suggests clinical disorders or settings where the test may be helpful
Assessing thrombopoietic activity with increased values observed when bone marrow is stimulated to produce platelets
Differentiating causes of thrombocytopenia
Predicting platelet recovery in patients undergoing treatments that affect platelet counts, such as chemotherapy
Method Name
A short description of the method used to perform the test
Flow Cytometry
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
Reticulocyted platelets
Immature PLT fraction
IPF%
Specimen Type
Describes the specimen type validated for testing
Whole Blood EDTA
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
0.5 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
| Clotted | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 48 hours | |
| Ambient | 24 hours |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Assessing thrombopoietic activity with increased values observed when bone marrow is stimulated to produce platelets
Differentiating causes of thrombocytopenia
Predicting platelet recovery in patients undergoing treatments that affect platelet counts, such as chemotherapy
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Immature platelets or reticulated platelets are newly released thrombocytes. They can be identified by their large size and high RNA concentration in their cytoplasm. Immature platelet fraction (IPF) represents the number of immature platelets compared to the total number of platelets as a percentage.(1)
Immature platelet fraction is a direct cellular measurement of thrombopoiesis. It can be used to differentiate causes of thrombocytopenia. IPF can be low or normal in the setting of decreased platelet production (hypoproductive) and increased in the setting of platelet destruction (hyperdestructive/consumptive).(1)
Immature platelet fraction increases prior to platelet recovery after chemotherapy and after stem cell transplantation by 2 to 3 days. This can prevent unnecessary platelet transfusion and provide an indicator for a successful engraftment after both autologous and allogeneic stem cell transplantation.(2)
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
1.1-8.6%
Interpretation
Provides information to assist in interpretation of the test results
Results that increase from baseline or that are above the established reference intervals indicate increased thrombopoiesis. Values lower than normal may indicate decreased thrombopoiesis.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Transfusions have been shown to lower immature platelet fraction percentage, either as a result of suppression of thrombopoiesis (negative feedback on thrombopoietin), due to dilution, or both.(1)
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Benlachgar N, Doghmi K, Masrar A, Mahtat EM, Harmouche H, Mezalek ZT. Immature platelets: a review of the available evidence. Thromb Res. 2020;195:43-50
2. Yang T, Tsai C, Wang H, Ko P, Shien S, Lin T, et al. Early prediction of platelet recovery with immature platelet fraction in patients receiving hematopoietic stem cell transplantation. Ann Hematol. 2024;103(11):4661-4670
Method Description
Describes how the test is performed and provides a method-specific reference
The Sysmex XN platelet fluorescent (PLT-F) analysis is based on flow cytometry method using a semiconductor laser, a two-dimensional scattergram is plotted, with the X-axis representing the intensity of the side fluorescent light, and the Y-axis representing the intensity of the forward scattered light. The scattergram displays groups of platelets, part of red blood cells, part of white blood cells, and debris.
The immature platelet fraction (IPF) is obtained as a ratio of platelet count in the area with strong fluorescent light intensity in the PLT-F scattergram (IPF zone), to the total platelet count.(Instruction manual: Automated Hematology Analyzer XN series [XN-9000/XN-9100] North American Edition. Code No. BF691913 en-am. Sysmex Corp; rev: 09/2022)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Sunday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
85055
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| IMPLF | Immature Platelet Fraction, B | 71693-6 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| IPF | Immature Platelet Fraction | 71693-6 |
| IPFRE | IPF Trigger Retic | No LOINC Needed |
| JIMPL | CBC Type | No LOINC Needed |