Diagnosis of multiple sclerosis; especially useful in patients with equivocal clinical presentation and radiological findings
Only orderable as part of a profile. For more information, see:
-OLIG / Oligoclonal Banding, Serum and Spinal Fluid
-MSP3 / Multiple Sclerosis (MS) Cascade, Serum and Spinal Fluid
Isoelectric Focusing (IEF) with IgG Immunoblot Detection
Multiple Sclerosis (MS)
Oligoclonal Banding
CSF
Only orderable as part of a profile. For more information, see:
-OLIG / Oligoclonal Banding, Serum and Spinal Fluid
-MSP3 / Multiple Sclerosis (MS) Cascade, Serum and Spinal Fluid
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Label specimen as spinal fluid.
See Specimen Required
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
CSF | Ambient | 14 days | |
Refrigerated (preferred) | 14 days | ||
Frozen | 14 days |
Diagnosis of multiple sclerosis; especially useful in patients with equivocal clinical presentation and radiological findings
The diagnosis of multiple sclerosis (MS) is dependent on clinical, radiological, and laboratory findings. The detection of increased intrathecal immunoglobulin (Ig) synthesis is the basis for current diagnostic laboratory tests for MS. These tests include the cerebrospinal fluid (CSF) IgG index and CSF oligoclonal band (OCB) detection. Abnormal CSF IgG indexes and OCB patterns have been reported in 70% to 80% of MS patients.
Increased intrathecal Ig synthesis may occur in other inflammatory CSF diseases and, therefore, this assay is not specific for MS.
Only orderable as part of a profile. For more information, see:
-OLIG / Oligoclonal Banding, Serum and Spinal Fluid
-MSP3 / Multiple Sclerosis (MS) Cascade, Serum and Spinal Fluid
Cerebrospinal fluid Oligoclonal Bands Interpretation: <2 bands
When the oligoclonal band assay detects 2 or more unique IgG bands in the cerebrospinal fluid (CSF), the result is positive.
Cerebrospinal fluid is used in the diagnosis of multiple sclerosis (MS) by identifying increased intrathecal IgG synthesis qualitatively (oligoclonal bands). The presence of 2 or more unique CSF oligoclonal bands was reintroduced as one of the diagnostic criteria for MS in the 2017 revised McDonald criteria. These findings however, are not specific for MS as CSF-specific IgG synthesis may also be found in patients with other neurologic diseases including infectious, inflammatory, cerebrovascular, and paraneoplastic disorders. Clinical correlation recommended.
These tests are not specific for multiple sclerosis.
1. Andersson M, Alvarez-Cermeno J, Bernardi G, et al. Cerebrospinal fluid in the diagnosis of multiple sclerosis: a consensus report. J Neurol Neurosurg Psychiatry. 1994;57(8):897-902
2. Fortini AS, Sanders EL, Weinshenker BG, Katzmann JA. Cerebrospinal fluid oligoclonal bands in the diagnosis of multiple sclerosis. Isoelectric focusing with the IgG immunoblotting compared with high resolution agarose gel electrophoresis and cerebrospinal fluid IgG index. Am J Clin Pathol. 2003:120(5):672-675
3. Thompson AJ, Banwell BL, Barkhof F, et al. Diagnosis of multiple sclerosis: 2017 revisions of the McDonald criteria. Lancet Neurol. 2018;17(2):162-173
4. Gurtner KM, Shosha E, Bryant SC, et al. CSF free light chain identification of demyelinating disease: comparison with oligoclonal banding and other CSF indexes. Clin Chem Lab Med. 2018;56(7):1071-1080
5. Saadeh R, Pittock S, Bryant S, et al. CSF kappa free light chains as a potential quantitative alternative to oligoclonal bands in multiple sclerosis. Neurology. 2019;92(15_supplement). doi:10.1212/WNL.92.15_supplement.S37.001
The oligoclonal band (OCB) assay requires paired cerebrospinal fluid (CSF) and serum samples. Unconcentrated CSF and diluted serum are electrophoresed by isoelectric focusing. The separated immunoglobulins (Ig) are visualized by an IgG immunoblot, and OCBs that are present in the CSF and not in the serum are reported. The assay uses reagents from Helena Laboratories.(Package insert: SPIFE Touch IgG IEF Procedure. Helena Laboratories. 02/2024)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
83916
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
OLIGC | CSF Bands | 100756-6 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
8017 | CSF Bands | 49852-7 |
23611 | CSF Olig Bands Interpretation | 100756-6 |