Test Id : IDENT
Organism Referred for Identification, Aerobic Bacteria
Useful For
Suggests clinical disorders or settings where the test may be helpful
Identification of pure isolates of aerobic bacteria
Differentiation of members of the Staphylococcus aureus complex (S aureus, Staphylococcus argenteus, Staphylococcus singaporensis, and Staphylococcus roterodami)
Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.
| Test Id | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, the reflex tests may be performed at an additional charge. All aerobically growing bacteria submitted will be identified and billed, as appropriate.
Method Name
A short description of the method used to perform the test
Dependent on organism submitted, 1 or more of the following methods will be used: Conventional Biochemical Testing, Commercial Identification Strips or Panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry, and 16S RNA Gene Sequencing
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
50012-IDENT
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, the reflex tests may be performed at an additional charge. All aerobically growing bacteria submitted will be identified and billed, as appropriate.
Specimen Type
Describes the specimen type validated for testing
Varies
Ordering Guidance
Mayo Clinic Laboratories will not perform identification testing on suspected select agents (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis). Consult with your state health department or the Centers for Disease Control and Prevention regarding identification confirmation or exclusion of such isolates. For more information see www.selectagents.gov/sat/list.htm.
Additional Testing Requirements
If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibilities are not appropriate and not performed, ZMMLS will be canceled.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines for shipping information.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
1. Specimen source (anatomical body site) is required.
2. Isolate description is required including: Gram stain reaction, morphology, and tests performed.
ORDER QUESTIONS AND ANSWERS
| Question ID | Description | Answers |
|---|---|---|
| Q00M0032 | Specimen Source (Required) and Isolate Description-Gram reaction, morphology, tests performed (Required) |
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Preferred:
Specimen Type: Bacterial isolate swab
Supplies:
E-swab (T853)
Infectious Container, Large (T146)
Container/Tube:
Preferred: E-Swab collection and transport system
Acceptable: Flocked swab and 1-mL liquid Amies transport medium in 12 x 80 mm tube
Collection Instruction:
1. Perform isolation of infecting bacteria.
2. Utilize the flocked swab to obtain an adequate sample of pure cultured isolate. Do not submit mixed cultures.
3. Place swab into the transport system containing 1-mL liquid Amies transport medium.
4. If needed, break off end of swab and close the transport tube.
5. Place the transport system into the secondary infectious container for shipment.
6. Each isolate must be submitted under a separate order.
Note: For the following organisms, submit an agar slant or other appropriate media to ensure viability upon arrival to the laboratory; Neisseria gonorrhoeae, Campylobacter sp., Helicobacter pylori and any other fastidious organism.
Acceptable:
Specimen Type: Pure culture of organism from source cultured
Supplies: Infectious Container, Large (T146)
Container/Tube: Agar slant or other appropriate media
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
3. Place the agar slant or other appropriate media into the secondary infectious container for shipment.
4. Each isolate must be submitted under a separate order.
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
See Specimen Required
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Other | Agar plate |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Useful For
Suggests clinical disorders or settings where the test may be helpful
Identification of pure isolates of aerobic bacteria
Differentiation of members of the Staphylococcus aureus complex (S aureus, Staphylococcus argenteus, Staphylococcus singaporensis, and Staphylococcus roterodami)
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, the reflex tests may be performed at an additional charge. All aerobically growing bacteria submitted will be identified and billed, as appropriate.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Organisms are referred to confirm identification or when the identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.
Techniques employed may include conventional biochemical analysis, commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry or sequencing nucleic acid of the 16S ribosomal RNA gene.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Identification of organism
Interpretation
Provides information to assist in interpretation of the test results
Genus and species are reported on aerobic bacterial isolates, whenever possible.
Bacillus species will be reported as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless species identification is specifically requested on the request form.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Isolates suspected of being select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) should be submitted to client's state health department or the Centers for Disease Control and Prevention for identification confirmation or exclusion.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Carroll KC, Pfaller MA, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019
2. Procop GW, Church DL, Hall GD, et al, eds. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Lippincott Williams and Wilkins; 2017
Method Description
Describes how the test is performed and provides a method-specific reference
Pure isolates of aerobic organisms received on slants are inoculated onto culture plates based on Gram stain morphology, the source of the isolate, clinical history, and previous results submitted by the referring client. In general, the utilized routine media include chocolate blood agar, sheep blood agar, and eosin methylene blue agar. After incubation at 35 degrees C in 5% carbon dioxide, the organism is identified using one or a combination of the following techniques: commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, conventional biochemical tests, carbon source utilization, and nucleic acid sequencing of the 16S ribosomal RNA gene.(JH Jorgensen, MA Pfaller, KC Carrol, et al. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015; Weyant RS, Moss CW, Weaver RE, et al. Identification of Unusual Pathogenic Gram-negative Aerobic and Facultatively Anaerobic Bacteria. Williams and Wilkins; 1996; Krieg NR, ed. Bergey's Manual of Systematic Bacteriology. Vol 1. Williams and Wilkins, 1984; Kolbert CP, Persing DH. Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol. 1999;2[3]:299-305)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
87077-Organism Referred for Identification, Aerobic Bacteria
87077-Identification Commercial Kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria Identification (if appropriate)
87153-Aerobe Ident by Sequencing (if appropriate)
87077-Additional Identification Procedure (if appropriate)
87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)
87147-Serologic Agglut Method 2 Ident (if appropriate)
87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)
87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87798-Identification by PCR (if appropriate)
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| IDENT | Organism Refer for ID, Aerobic Bact | 32367-5 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| IDENT | Organism Refer for ID, Aerobic Bact | In Process |