Test Id : PPAP
Parental Sample Prep for Prenatal Microarray Testing, Blood
Useful For
Suggests clinical disorders or settings where the test may be helpful
Preparing parental blood specimen for possible confirmation testing if an abnormality is detected on the prenatal array sample
DNA extraction of the maternal blood specimen used for maternal cell contamination testing
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.
When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.
Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.
Method Name
A short description of the method used to perform the test
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.
When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.
Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.
Specimen Type
Describes the specimen type validated for testing
Whole blood
Additional Testing Requirements
This test must be ordered in conjunction with CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue. Each parent should have a specimen collected for this test ID using a different order number than the fetal specimen collected for the CMAP, CMAPC, or CMAMT order.
Necessary Information
1. The reason for testing is required.
2. Include the name listed on the prenatal specimen that was submitted for CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
This test requires 2 blood specimens: 1 sodium heparin and 1 EDTA
Specimen Type: Whole blood
Container/Tube: Green top (sodium heparin) and lavender top (EDTA)
Specimen Volume: 3 mL EDTA and 4 mL sodium heparin
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimens in original tubes. Do not aliquot.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
2 mL EDTA and 2 mL sodium heparin whole blood
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Ambient (preferred) | ||
| Refrigerated | |||
Useful For
Suggests clinical disorders or settings where the test may be helpful
Preparing parental blood specimen for possible confirmation testing if an abnormality is detected on the prenatal array sample
DNA extraction of the maternal blood specimen used for maternal cell contamination testing
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.
When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.
Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
In order to interpret equivocal array results on a prenatal sample (amniotic fluid or chorionic villus), parental studies are performed to determine if the abnormality detected on the prenatal array is inherited or de novo.
Maternal cell contamination testing is performed on the maternal blood and prenatal specimen to detect the presence of maternal cells in the fetal sample.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
An interpretive report will be provided.
Interpretation
Provides information to assist in interpretation of the test results
No interpretation will be provided. This test is for specimen processing only.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
No significant cautionary statements
Method Description
Describes how the test is performed and provides a method-specific reference
The heparinized sample will be cultured following standard laboratory protocols and a fixed-cell pellet will be prepared. DNA will be extracted from the EDTA sample and used to perform maternal cell contamination testing on the maternal sample. The fixed cell pellet and DNA will be stored in the laboratory for possible confirmation testing if an abnormality is detected on the prenatal array sample.(Unpublished Mayo method)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
Not Applicable
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
This test ID contains no charge and serves as a way to correlate proband parental specimens. If additional testing is warranted, the appropriate tests will be added.
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| PPAP | Parental Prenatal Array Prep Test | In Process |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| 55079 | Result Summary | 50397-9 |
| 52978 | Interpretation | 69965-2 |
| 52979 | Reason For Referral | 42349-1 |
| 53337 | Specimen | 31208-2 |
| 53338 | Source | 31208-2 |
| 53339 | Method | 85069-3 |