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Test Catalog

Test ID: FC13A    
Factor 13a Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the identification of acquired digital fibrokeratomas, angiofibromas, and oral fibroma, and a proportion of cells in histiocytomas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Factor XIIIa, a blood and intracellularly produced coagulation factor, has been found in a variety of cell types, including fibroblast-like mesenchymal cells, and has been shown to stimulate the proliferation of fibroblasts and neoplastic cells in vitro. Immunohistochemical staining for factor XIIIa labels normal dermal dendrocytes, the large stellate fibroblasts found in acquired digital fibrokeratomas, angiofibromas, and oral fibroma, and a proportion of cells in histiocytomas. Factor XIIIa immunostain also produces cytoplasmic staining of dermal dendrocytes in normal skin and of a proportion of cells in histiocytomas.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. West KL, Cardona DM, Su Z, Puri PK: Immunohistochemical markers in fibrohistiocytic lesions: factor XIIIa, CD34, S-100 and p75. Am J Dermatopathol. 2014 May;36(5):414-419. doi: 10.1097/DAD.0b013e3182a70396

2. de Alvarenga Lira ML, Pagliari C, de Lima Silva AA, de Andrade HF Jr, Duarte MI: Dermal dendrocytes FXIIIa+ are essential antigen-presenting cells in indeterminate leprosy. Am J Dermatopathol. 2015 Apr;37(4):269-273. doi: 10.1097/DAD.0000000000000238

3. Hirai KE, Aarao TL, Silva LM, et al: Langerhans cells (CD1a and CD207), dermal dendrocytes (FXIIIa) and plasmacytoid dendritic cells (CD123) in skin lesions of leprosy patients. Microb Pathog. 2016 Feb;91:18-25. doi: 10.1016/j.micpath.2015.11.013