TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: CD8I    
CD8 Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Aids in the identification of cytotoxic T cells

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

CD8 is expressed in a subset of T cells (cytotoxic T cells). It appears on the surface of cytotoxic T lymphocytes during intrathymic maturation and is present on approximately 20% of the T cells in the peripheral blood, lymph nodes, and spleen.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Chu PG, Arber DA, Weiss LM: Expression of T/NK-cell and plasma cell antigens in non hematopoietic epithelioid neoplasms. An Immunohistochemical Study of 447 Cases. Am J Clin Pathol 2003;120:64-70

2. Higgins RA, Blankenship JE, Kinney MC: Application of immunohistochemistry in the diagnosis of non-Hodgkin and Hodgkin lymphoma. Archives of Pathology and Laboratory Medicine 2008;132(3):441-461

3. Oudejans JJ, van der Valk P: Immunohistochemical classification of T cell and NK cell neoplasms. J Clin Pathol 2002;55(2):892