Test Catalog

Test ID: CD43    
CD43 Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Identification of T lymphocytes, monocytes, macrophages, granulocytes, plasma cells, and a subset of B lymphocytes

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

CD43 (cluster of differentiation 43) is expressed by T lymphocytes and myeloid cells including granulocytes and precursors, monocytes, macrophages, histiocytes, plasma cells and megakaryocytes. In normal tonsil, CD43 will mainly show staining in T lymphocytes, histiocytes and plasma cells.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation; only technical performance of the stain. If an interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Zargaran M, Baghaei F, Moghimbeigi A, Baghai F: Explanation for different behavior of peripheral and central giant cell granuloma by CD44 and CD34 immunostaining. J Investig Clin Dent. 2019 Nov;10(4):e12451. doi: 10.1111/jicd.12451

2. Xiong B, Nie Y, Tang Z, Xue M, Zuo X: Prognostic evaluation of ALIP and CD34 immunostaining in IPSS-R subgroups of myelodysplastic syndromes. Pathology. 2017 Aug;49(5):526-533. doi: 10.1016/j.pathol.2017.05.001

3. Sardina LA, Piliang M, Bergfeld WF: Diagnostic value of CD34 and calretinin immunostaining in the diagnosis of proliferating tricholemmal tumor and trichoblastoma. Int J Dermatol. 2019 Apr 23. doi: 10.1111/ijd.14461