Test Catalog

Test ID: CD23    
CD23 Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Identification of follicular dendritic cells


Classification of low-grade B-cell lymphomas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

CD23 strongly stains the cytoplasm and membranes of follicular dendritic cells and the membranes of a subset of follicular mantle zone B-lymphocytes. Typically, B-cell small lymphocytic lymphoma/chronic lymphocytic leukemias are CD5 positive and CD23 positive, while mantle cell lymphoma is CD5 positive and CD23 negative. Antibodies to CD23 are diagnostically useful in the classification of low-grade B-cell lymphomas.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation: only technical performance of the stain.If an interpretation is required, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. van den Brand M, van Krieken JH: Small cell B-cell lymphoma. Diagn Histopathol. 2015;21(10):383-390

2. Zhang XM, Aguilera N: New immunohistochemistry for B-cell lymphoma and Hodgkin lymphoma. Arch Pathol Lab Med. 2014;138(12):1666-1672

3. Gualco G, Natkunam Y, Bacchi CE: The spectrum of B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma and classical Hodgkin lymphoma: a description of 10 cases. Mod Pathol. 2012;25(5):661-674