Test Catalog

Test Id : FROS2

Fructose, Qualitative, Semen

Useful For
Suggests clinical disorders or settings where the test may be helpful

Establishing the origin of azoospermia in patients with azoospermia and low volume ejaculates

Method Name
A short description of the method used to perform the test


NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Qualitative Fructose,Semen

Lists additional common names for a test, as an aid in searching


Specimen Type
Describes the specimen type validated for testing


Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection.


Submit only 1 of the following specimens:


Specimen Type: Semen

Collection Container/Tube: Sterile container

Submission Container/Tube: Plastic container

Specimen Volume: Total ejaculate

Collection Instructions: Do not dilute specimen. Freeze specimen at -20 degrees C.


Specimen Type: Seminal plasma

Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. After semen collection, wait 30 to 40 minutes until the semen is liquefied, then centrifuge the semen for 10 minutes at maximum centrifuge speed.

2. Remove top 3/4 of specimen with a pipet and place in a plastic vial. Freeze specimen at -20 degrees C.

3. Discard remainder of centrifuged specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Semen Frozen

Useful For
Suggests clinical disorders or settings where the test may be helpful

Establishing the origin of azoospermia in patients with azoospermia and low volume ejaculates

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Fructose is produced in the male reproductive tract by the seminal vesicles and is released into the semen during ejaculation. Fructose is the energy source for sperm motility.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


Provides information to assist in interpretation of the test results

A positive (indicated by color change) fructose is considered normal.


A semen specimen that contains no sperm (azoospermia) and is fructose negative may indicate an absence of the seminal vesicles, absence of the vas deferens in the area of the seminal vesicles, or an obstruction at the level of the seminal vesicles.

Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

This test should be performed in conjunction with a semen analysis to determine semen volume, pH, sperm concentration, motility, and grade of forward progression.


Fructose test must be on a separate ejaculate.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Lipshultz LI, Howards SS, Niederberger CS, eds: In: Infertility in the Male. 4th ed. Cambridge University Press 2009

2. Risz B, Agarwal A, Sabanegh ES, eds: Male Infertility in Reproductive Medicine: Diagnosis and Management. CRC Press; 2019

Method Description
Describes how the test is performed and provides a method-specific reference

A qualitative method using resorcinol will detect the presence or absence of fructose.(Keel BA, Webster BW: Handbook of the Laboratory Diagnosis and Treatment of Infertility. CRC Press: 1990:49)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.


Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 7 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Not retained

Performing Laboratory Location
Indicates the location of the laboratory that performs the test


Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.


LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
FROS2 Qualitative Fructose,Semen 13943-6
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
FRUCS Fructose, Semen/Seminal P 13943-6
CLSM Collection Site 56816-2
VOLSM Semen Volume 3160-9
CMT49 Comment 48767-8

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports