Test Catalog

Test Id : FFTRP

Trofile Co-Receptor Tropism Assay

Useful For
Suggests clinical disorders or settings where the test may be helpful

To determine the co-receptor tropism (CCR5, CXCR4, or dual/mixed) of a patient's HIV-1 strain for selection of CCR5 co-receptor antagonist therapy, when a patient's HIV-1 viral load is > or = 1,000 copies/mL. 

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

CD4 cell culture assay for phenotypic recombinant-virus co-receptor tropism.

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Trofile Phenotypic Corecept Tropism

Aliases
Lists additional common names for a test, as an aid in searching

FTRPC

CCR5, CXCR4, CR5

Chemokine co-receptor

Selzentry, Maraviroc

Trofile

Tropism

Tropotype

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Specimen Type
Describes the specimen type validated for testing

Plasma EDTA

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
Z2092 Most Recent Viral Load
Z2093 Viral Load Collected Date

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Draw blood in either PPT (pearl top) or lavender-top (EDTA) tubes. Remove plasma from cells immediately, and transfer specimen to a screw-capped, plastic vial. Freeze 3 mL of PPT plasma or EDTA plasma immediately, send specimen frozen.

 

RECOMMENDED:

1.       Patient's most recent viral load

2.       Viral load collection date

 

Note:   1.  Intended to use only for patients with viral loads greater than or equal to 1000 copies/mL. For best results, viral loads should be confirmed within two weeks prior to submission for testing at Monogram.

           2. Patient samples submitted <30 days apart are considered duplicate and will be cancelled.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis NA
Lipemia NA
Icterus NA
Other NA

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 14 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

To determine the co-receptor tropism (CCR5, CXCR4, or dual/mixed) of a patient's HIV-1 strain for selection of CCR5 co-receptor antagonist therapy, when a patient's HIV-1 viral load is > or = 1,000 copies/mL. 

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

 

Interpretation
Provides information to assist in interpretation of the test results

CCR5 Tropic (R5) HIV-1

Virus uses CCR5 to enter CD4+ cells.

 

CXCR4 Tropic (X4) HIV-1

Virus uses CXCR4 to enter CD4+ cells

 

DUAL/MIXED Tropic (D/M) HIV-1

Dual-tropic viruses can use either CCR5 or CXCR4 to enter CD4+ cells. Mixed-tropic populations contain viruses with two or more tropisms.

 

Non-reportable

Co-receptor tropism could not be determined by the Trofile assay. Common causes of a non-reportable result are viral load <1,000 copies/mL, reduced viral fitness, or compromised sample collection/handling.

 

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into

CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4.

 

Trofile is a cell-based approach to determine a patient’s HIV co-receptor tropism (or “Tropotype™). Trofile uses the complete gp160 coding region of the HIV-1 envelope protein ensuring that all of the determinants of tropism are tested. CLIA validation experiments demonstrate that Trofile is 100% sensitive at detecting 0.3% CXCR4-using minor variants.

 

This class of drugs binds to CCR5 and blocks CCR5-mediated HIV entry into host cells. Trofile is used to determine whether a CCR5 antagonist may be an appropriate drug for a patient. Several clinical trials on CCR5 antagonists have demonstrated the positive and negative predictive value of Trofile in clinical settings.

 

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

18 to 23 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Monogram Biosciences, Inc

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87999

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
FFTRP Trofile Phenotypic Corecept Tropism 57182-8
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
Z1038 Tropotype Result 57182-8
Z1039 Note: Not Provided
Z1040 Activity of CCR5 antag anticipated? Not Provided

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports