Test Id : FFTRP
Trofile Co-Receptor Tropism Assay
Useful For
Suggests clinical disorders or settings where the test may be helpful
Detect HIV-1 coreceptor tropism; determine eligibility for CCR5 antagonist therapy such as maraviroc (Selzentry)
Method Name
A short description of the method used to perform the test
Polymerase chain reaction (PCR) amplification and viral culture.
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
FTRPC
CCR5, CXCR4, CR5
Chemokine co-receptor
Selzentry, Maraviroc
Trofile
Tropism
Tropotype
Specimen Type
Describes the specimen type validated for testing
Plasma EDTA
ORDER QUESTIONS AND ANSWERS
| Question ID | Description | Answers |
|---|---|---|
| Z2092 | Most Recent Viral Load | |
| Z2093 | Viral Load Collected Date |
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Plasma
Specimen Volume: 3 mL
Collection Instructions: Draw blood in either PPT (pearl top) or lavender-top (EDTA) tubes.
Centrifuge specimen within six hours of collection. Remove plasma from cells immediately, and transfer specimen to a screw-capped, plastic vial. Freeze 3 mL of PPT plasma or EDTA plasma immediately, send specimen frozen.
To avoid delays in turnaround time when requesting multiple tests, please submit separate frozen specimens for each test requested.
RECOMMENDED:
1. Patient's most recent viral load
2. Viral load collection date
Note: 1. Intended to use only for patients with viral loads greater
2. Patient samples submitted <30 days apart are
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
1 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Other | PPT not centrifuged; insufficient volume; heparinized plasma; non frozen specimens |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 14 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Detect HIV-1 coreceptor tropism; determine eligibility for CCR5 antagonist therapy such as maraviroc (Selzentry)
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Interpretation
Provides information to assist in interpretation of the test results
CCR5 Tropic (R5) HIV-1
Virus uses CCR5 to enter CD4+ cells.
CXCR4 Tropic (X4) HIV-1
Virus uses CXCR4 to enter CD4+ cells
DUAL/MIXED Tropic (D/M) HIV-1
Dual-tropic viruses can use either CCR5 or CXCR4 to enter CD4+ cells. Mixed-tropic populations contain viruses with two or more tropisms.
Non-reportable
Co-receptor tropism could not be determined by the Trofile assay. Common causes of a non-reportable result are viral load <1,000 copies/mL, reduced viral fitness, or compromised sample collection/handling.
Method Description
Describes how the test is performed and provides a method-specific reference
Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into
CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4.
Trofile is a cell-based approach to determine a patient’s HIV co-receptor tropism (or
This class of drugs binds to CCR5 and blocks CCR5-mediated HIV entry into host cells. Trofile is used to determine whether a CCR5 antagonist may be an appropriate drug for a patient. Several clinical trials on CCR5 antagonists have
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Sunday through Saturday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
87999
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| FFTRP | Trofile Phenotypic Corecept Tropism | 57182-8 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| Z1038 | Tropotype Result | 57182-8 |
| Z1039 | Note: | Not Provided |
| Z1040 | Activity of CCR5 antag anticipated? | Not Provided |